Volume 22, Issue 2 (2019)
mjms 2019, 22(2): 91-95 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Dalimi A, Mohammadiha A, Mohammadnajjar M. A molecular study on visceral leishmaniasis and babesiosis in domestic dogs of Meshkinshahr, Ardabil Province. mjms 2019; 22 (2) :91-95
URL: http://mjms.modares.ac.ir/article-30-23735-en.html
URL: http://mjms.modares.ac.ir/article-30-23735-en.html
1- Parasitology & Entomology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , dalimi_a@modares.ac.ir
2- Parasitology & Entomology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Parasitology & Entomology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abstract: (6677 Views)
Aims: Visceral leishmaniasis (VL) is one of the most important endemic diseases in Meshkinshahr. The present study was aimed to molecular detect of VL and babesiosis in clot blood samples taken from dogs.
Materials and Methods: In this experimental study, a total number of 148 blood samples were collected from dogs with clinical signs of visceral leishmaniasis from Meshkinshahr area. All serum samples were examined by direct agglutination test (DAT). DNA was extracted from the blood clot with the DNG-plus Extraction Kit. The ITS1 and kDNA genes of L. infantum were amplified by the PCR method. To identify and confirm the existence of Babesia parasites, 2 pairs of Babesia-specific primers were used.
Results: All of 148 dogs (100%) showed titers more than 1:320 of L. infantum infection in the serological test. L. infantum kDNA was detected by PCR in all samples. No cases of infection with Babesia parasite found in the samples.
Conclusions: The dog owners in Meshkinshehr are in close contact with dogs which are positive for VL, so, the implementation of health care in this region is highly recommended.
Materials and Methods: In this experimental study, a total number of 148 blood samples were collected from dogs with clinical signs of visceral leishmaniasis from Meshkinshahr area. All serum samples were examined by direct agglutination test (DAT). DNA was extracted from the blood clot with the DNG-plus Extraction Kit. The ITS1 and kDNA genes of L. infantum were amplified by the PCR method. To identify and confirm the existence of Babesia parasites, 2 pairs of Babesia-specific primers were used.
Results: All of 148 dogs (100%) showed titers more than 1:320 of L. infantum infection in the serological test. L. infantum kDNA was detected by PCR in all samples. No cases of infection with Babesia parasite found in the samples.
Conclusions: The dog owners in Meshkinshehr are in close contact with dogs which are positive for VL, so, the implementation of health care in this region is highly recommended.
References
1. Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I, Arshi S, Zarei Z, et al. Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet Parasitol. 2005;129(3-4):243-51. [Link] [DOI:10.1016/j.vetpar.2005.01.010]
2. Mohebali M, Edrissian GH, Shirzadi MR, Akhoundi B, Hajjaran H, Zarei Z, et al. An observational study on the current distribution of visceral leishmaniasis in different geographical zones of Iran and implication to health policy. Travel Med Infect Dis. 2011;9(2):67-74. [Link] [DOI:10.1016/j.tmaid.2011.02.003]
3. Gavgani AS, Mohite H, Edrissian GH, Mohebali M, Davies CR. Domestic dog ownership in Iran is a risk factor for human infection with Leishmania infantum. Am J Trop Med Hyg. 2002;67(5):511-5. [Link] [DOI:10.4269/ajtmh.2002.67.511]
4. Molaei S, Dalimi A, Mohebali M, Zareii Z, Mohammadi-Ghalehbin B, Akhondi B, Azarm A. Study of Canine Visceral Leishmaniasis in Symptomatic and Asymptomatic Domestic Dogs in Meshkinshahr City, Iran. J Ardabil Univ Med Sci. 2016;16(1):105-15. [Persian] [Link]
5. Harith A, Salappendel RJ, Reiter I, Knapen F, Korte P, Huigen E, et al. Application of a direct agglutination test for detection of specific anti-leishmania antibodies in the canine reservoir. J Clin Microbiol. 1998;27(10):2252-7. [Link]
6. Mohebali M, Edrissian GhH, Nadim A, Hajjaran H, Akhoundi B, Hooshmand B, et al. Application of direct agglutination test (DAT) for the diagnosis and seroepidemiological studies of visceral leishmaniasis in Iran. Iran J Parasitol. 2006;1(1):15-25. [Link]
7. El Tai N, Osman OF, El Fari M, Presber W, Schönian G. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg. 2000;94(5):575-9. [Link] [DOI:10.1016/S0035-9203(00)90093-2]
8. Mary C, Faraut F, Lascombe L, Dumon H. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol. 2004;42(11):5249-55. [Link] [DOI:10.1128/JCM.42.11.5249-5255.2004]
9. Carret C, Walas F, Carcy B, Grande N, Précigout E, Moubri K, et al. Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: Differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. J Eukaryot Microbiol. 1999;46(3):298-303. [Link] [DOI:10.1111/j.1550-7408.1999.tb05128.x]
10. Ansari H, Moshfe AA , Solhjoo K, Khodadadi P , Kalantari M, Afshoon E, et al. Characterization of visceral leishmaniasis in reservoir host (dogs) and determination of agent by PCR in Boyer-Ahmad District, Iran. Armaghane-danesh. 2012;16(6):537-44. [Persian] [Link]
11. Moshfe AA, Mohebali M, Edrissian GH, Zarei Z , Akhoundi B, Kazemi B, et al. Canine visceral leishmaniasis: Asymptomatic infected dogs as a source of L. infantum infection. Acta Trop. 2009;112(2):101-5. [Link] [DOI:10.1016/j.actatropica.2009.07.004]
12. Khanmohammadi M, Fallah E, Rahbari S, Sohrabi I, Farshchian M, Hamzavi F, et al. Study on Seroprevalence of Canine Visceral Leishmaniasis (CVL) in Ownership Dogs of Sarab, East Azerbaijan, Province, Northwest of Iran with Indirect Immuno Fluorescence Antibody Test (IFAT) and its Health Importance in 2008-2009. J Anim Vet Adv. 2010;9(1):139-43. [Link] [DOI:10.3923/javaa.2010.139.143]
13. Mohammadi-Ghalehbin B, Hatam GhR, Sarkari B, Mohebali M, Zarei Z, Jaberipour M, et al. Leishmania infantum FML-ELISA for the detection of symptomatic and asymptomatic canine visceral leishmaniasis in an endemic area of Iran. Iran J Immunol. 2011;8(4):244-25. [Link]
14. Barati M, Mohebali M, Alimohammadian MH, Khamesipour A, Akhoundi B, Zarei Z. Canine visceral leishmaniasis: Seroprevalence survey of asymptomatic dogs in an endemic area of northwestern Iran. J Parasit Dis. 2015;39(2):221-4. [Link] [DOI:10.1007/s12639-013-0325-2]
15. Alborzi AR, Avizeh R, Mosallanejad B, Razi Jalali MH, Hamidi Nejat H, Taghipour R. Babesia infection in urban and rural dogs in Ahvaz district, southwest of Iran. Arch Razi Inst. 2013;68(1):37-42. [Link]
16. Hosseinzadeh Varjoy M, Ashrafi Helan, J, Salehi N, Bazmani A, Nematollahi A, Imani Baran A. A survey on Babesia infection in dogs of urban and rural regions of Tabriz city, Iran. Vet J. 2015;29(2): 56-63 [Link]
17. O'Dwyer LH, Lopes VVA, Rubini AS, Paduan KDS, Ribolla PEM. Babesia spp. infection in dogs from rural areas of Sao Paulo State, Brazil. Rev Bras Parasitol Vet Jaboticabal. 2009;18(2):23-6. [Link] [DOI:10.4322/rbpv.01802005]
18. Bashir NI, Chaudhry ZI, Ahmed S, Saeed MA. Epidemiological and vector identification studies on canine babesiosis. Pak Vet J. 2009;29(2):51-4. [Link]
19. Omudu EA, Bernard O, Ayashar JG. Epidemiological survey of canine babesiosis in Makurdi, Nigeria. Anim Res Inter. 2007;4(3):745-9. [Link] [DOI:10.4314/ari.v4i3.48685]
20. Bourdoiseau G. Canine babesiosis in France. Vet Parasitol. 2006;138(1-2):118-25. [Link] [DOI:10.1016/j.vetpar.2006.01.046]
21. Adaszeka L, Martinez AC, Winiarczyk S. The factors affecting the distribution of babesiosis in dogs in Poland. Vet Parasitol. 2011;181(2-4):160-5. [Link] [DOI:10.1016/j.vetpar.2011.03.059]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |