Volume 23, Issue 1 (2020)
mjms 2020, 23(1): 11-17 |
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Rajaei N, Habibian Dehkordi S, Rahimi Junqani R, Alijani M, Sasannejad N. Evaluation of Lethal Effect of Aqueous and Alcoholic Extracts of Glycyrrhiza glabra on the Emeria tenella Oocysts In Vitro. mjms 2020; 23 (1) :11-17
URL: http://mjms.modares.ac.ir/article-30-30356-en.html
URL: http://mjms.modares.ac.ir/article-30-30356-en.html
1- Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2- Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , habibian_dehkordi@yahoo.com
2- Basic Sciences Department, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , habibian_dehkordi@yahoo.com
Abstract: (2029 Views)
Aims: Coccidiosis is one of the most common infectious diseases in poultry that causes huge economic losses. Glycyrrhiza glabra is a medicinal plant that used traditionally. The aim of the present study was to evaluate the effect of aqueous and alcoholic extracts of this plant on the Emeria tenella oocysts in vitro.
Materials & Methods: The unsporulated oocysts were obtained by inoculation of 14th day-old broiler chicks with 75,000 oocysts. To obtain sporulated oocysts, 9g of feces samples was soaked in 2% potassium bichromate and incubated at 27°C for 72h. Aqueous and alcoholic extracts of Glycyrrhiza glabra in concentrations of 1, 2, and 5% were prepared and oocysts were exposed to these extracts for 48 hours. Thereafter, the number of sporulated and unsporulated oocysts were counted at 1, 12, 24, and 48 hours. In order to prevent any error, the experiments were repeated three times.
Findings: Both extracts of Glycyrrhiza glabra in all tested concentrations cause a significant reduction in the number of sporulated and unsporulated Eimeria tenella oocysts compared with control (p<0.05). The rate of inhibitory effect of extracts had a direct relationship with exposure time, and inhibition was continuously increased over time.
Conclusion: Alcoholic extracts of Glycyrrhiza glabra had a better effect than aqueous extracts. 5% alcoholic extract had the best effect. However, further studies are needed to find the best dose for the most anticoccidial effects and also to show its effects on other species of Eimeria and in animal models.
Materials & Methods: The unsporulated oocysts were obtained by inoculation of 14th day-old broiler chicks with 75,000 oocysts. To obtain sporulated oocysts, 9g of feces samples was soaked in 2% potassium bichromate and incubated at 27°C for 72h. Aqueous and alcoholic extracts of Glycyrrhiza glabra in concentrations of 1, 2, and 5% were prepared and oocysts were exposed to these extracts for 48 hours. Thereafter, the number of sporulated and unsporulated oocysts were counted at 1, 12, 24, and 48 hours. In order to prevent any error, the experiments were repeated three times.
Findings: Both extracts of Glycyrrhiza glabra in all tested concentrations cause a significant reduction in the number of sporulated and unsporulated Eimeria tenella oocysts compared with control (p<0.05). The rate of inhibitory effect of extracts had a direct relationship with exposure time, and inhibition was continuously increased over time.
Conclusion: Alcoholic extracts of Glycyrrhiza glabra had a better effect than aqueous extracts. 5% alcoholic extract had the best effect. However, further studies are needed to find the best dose for the most anticoccidial effects and also to show its effects on other species of Eimeria and in animal models.
References
1. Dalloul RA, Lillehoj HS. Poultry coccidiosis: Recent advancements in control measures and vaccine development. Expert Rev Vaccines. 2006;5(1):143-63. [Link] [DOI:10.1586/14760584.5.1.143]
2. Abbas RZ, Iqbal Z, Blake D, Khan MN, Saleemi MK. Anticoccidial drug resistance in fowl coccidia: The state of play revisited. World's Poult Sci J. 2011;67(2):337-50. [Link] [DOI:10.1017/S004393391100033X]
3. Olejnik M, Szprengier-Juszkiewicz T. Coccidiostat residues in poultry tissues and eggs. Medycyna Weterynaryjna. 2007;63(12):1539-45. [Polish] [Link]
4. Hashemi SR, Davoodi H. Phytogenics as new class of feed additive in poultry industry. J Anim Vet Adv. 2010;9(17):2295-304. [Link] [DOI:10.3923/javaa.2010.2295.2304]
5. Abbas RZ, Iqbal Z, Khan MN, Zafar MA, Zia MA. Anticoccidial activity of Curcuma longa L. in broilers. Braz Arch Biol Technol. 2010;53(1):63-7. [Link] [DOI:10.1590/S1516-89132010000100008]
6. Chandrakesan P, Muralidharan K, Dinesh Kumar V, Ponnudurai G, Jeyagopal Harikrishnan T, Senthil Veland Natarajan Rani K. Efficacy of a herbal complex against caecal coccidiosis in broiler chickens. Veterinarski Arhiv. 2009;79(2):199-203. [Link]
7. Haq IU, Pasha TN, Khalique A. Comparative efficacy of herbal and allopathy drugs against coccidiosis in poultry. Ital J Anim Sci. 2011;10(1):e3. [Link] [DOI:10.4081/ijas.2011.e3]
8. Lee SH, Jang SI, Kim DK, Ionescu C, Bravo D, Lillehoj HS. Effect of dietary Curcuma, Capsicum, and Lentinus on enhancing local immunity against Eimeria acervulina infection. J Poult Sci. 2010;47(1):009025. [Link] [DOI:10.2141/jpsa.009025]
9. Youn HJ, Noh JW. Screening of the anticoccidial effects of herb extracts against Eimeria tenella. Vet Parasitol. 2001;96(4):257-63. [Link] [DOI:10.1016/S0304-4017(01)00385-5]
10. Eddouks M, Chattopadhyay D, De Feo V, Cho WC. Medicinal plants in the prevention and treatment of chronic diseases. Evid Based Complement Altern Med. 2012;2012:458274. [Link] [DOI:10.1155/2012/458274]
11. Li W, Asada Y, Yoshikawa T. Flavonoid constituents from Glycyrrhiza glabra hairy root cultures. Phytochemistry. 2000;55(5):447-56. [Link] [DOI:10.1016/S0031-9422(00)00337-X]
12. Mori K, Sakai H, Suzuki S, Akutsu Y, Ishikawa M, Imaizumi M, et al. Effects of glycyrrhizin (SNMC: Stronger Neo-Minophagen C®) in hemophilia patients with HIV-1 infection. Tohoku J Exp Med. 1990;162(2):183-93. [Link] [DOI:10.1620/tjem.162.183]
13. Chang CZ, Wu SC, Kwan AL. Glycyrrhizin attenuates proinflammatory cytokines through a peroxisome proliferator-activated receptor-γ-dependent mechanism and experimental vasospasm in a rat model. J Vasc Res. 2015;52(1):12-21. [Link] [DOI:10.1159/000381099]
14. Huang WF, Ouyang S, Li SY, Lin YF, Ouyang H, Zhang H, et al. Effect of quercetin on colon contractility and L-type Ca2+ channels in colon smooth muscle of guinea-pig. Acta Physiol Sin. 2009;61(6):567-76. [Link]
15. Liu B, Yang J, Wen Q, Li Y. Isoliquiritigenin, a flavonoid from licorice, relaxes guinea-pig tracheal smooth muscle in vitro and in vivo: role of cGMP/PKG pathway. Eur J Pharmacol. 2008;587(1-3):257-66. [Link] [DOI:10.1016/j.ejphar.2008.03.015]
16. Khayyal MT, El-Ghazaly MA, Kenawy SA, Seif-El-Nasr M, Mahran LG, Kafafi YA, et al. Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination. Drug Res. 2001;51(7):545-53. [Link] [DOI:10.1055/s-0031-1300078]
17. Tai CJ, Wang WC, Wang CK, Wu CH, Yang MD, Chang YJ, et al. Fermented wheat germ extract induced cell death and enhanced cytotoxicity of cisplatin and 5-fluorouracil on human hepatocellular carcinoma cells. Evid Based Complement Altern Med. 2013;2013:121725. [Link] [DOI:10.1155/2013/121725]
18. Gupta VK, Fatima A, Faridi U, Negi AS, Shanker K, Kumar JK, et al. Antimicrobial potential of Glycyrrhiza glabra roots. J Ethnopharmacol. 2008;116(2):377-80. [Link] [DOI:10.1016/j.jep.2007.11.037]
19. Krausse R, Bielenberg J, Blaschek W, Ullmann U. In vitro anti-Helicobacter pylori activity of Extractum liquiritiae, glycyrrhizin and its metabolites. J Antimicrob Chemother. 2004;54(1):243-6. [Link] [DOI:10.1093/jac/dkh287]
20. Saxena S. Glycyrrhiza glabra: Medicine over the millennium. Nat Prod Rad. 2005;4(5):358-67. [Link]
21. Badam L. In vitro antiviral activity of indigenous glycyrrhizin, licorice and glycyrrhizic acid (Sigma) on Japanese encephalitis virus. J Commun Dis. 1997;29(2):91-9. [Link]
22. Hoever G, Baltina L, Michaelis M, Kondratenko R, Baltina L, Tolstikov GA, et al. Antiviral Activity of glycyrrhizic acid derivatives against SARS- Coronavirus. J Med Chem. 2005;48(4):1256-9. [Link] [DOI:10.1021/jm0493008]
23. Ram A, Mabalirajan U, Das M, Bhattacharya I, Dinda AK, Gangal SV, et al. Glycyrrhizin alleviates experimental allergic asthma in mice. Int Immunopharmacol. 2006;6(9):1468-77.. [Link] [DOI:10.1016/j.intimp.2006.04.020]
24. Račková L, Jančinová V, Petríková M, Drábiková K, Nosáľ R, Štefek M, et al. Mechanism of anti-inflammatory action of liquorice extract and glycyrrhizin. Nat Product Res. 2007;21(14):1234-41. [Link] [DOI:10.1080/14786410701371280]
25. J Mason T, Chemat F, Vinatoru M. The extraction of natural products using ultrasound or microwaves. Curr Org Chem. 2011;15(2):237-47. [Link] [DOI:10.2174/138527211793979871]
26. Zaman MA, Iqbal Z, Abbas RZ, Khan MN. Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella. Parasitology. 2012;139(2):237-43. [Link] [DOI:10.1017/S003118201100182X]
27. Fudge AM. Laboratory medicine: avian and exotic pets. 1st Edition. Philadelphia: Saunders; 2000. [Link]
28. Beynen A, Gärtner K, Van Zutphen L. Principles of laboratory animal science. Philadelphia: Elsevier; 2003. [Link]
29. Yakhchali M, Khosravi AR. In vitro and in vivo assessment of plant essence (Artemisia Sieberl) coccidiocidal effect on rabbit coccidiosis. PAJOUHESH & SAZANDEGI. 2004;17(3):48-51. [Persian] [Link]
30. Conway DP, McKenzie ME. Poultry coccidiosis: Diagnostic and testing procedures. 3rd Edition. Hoboken: John Wiley & Sons; 2007. [Link] [DOI:10.1002/9780470344620]
31. Kumar P, Singh DK. Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and their active components against the snail Lymnaea acuminata. Chemosphere. 2006;63(9):1568-74. [Link] [DOI:10.1016/j.chemosphere.2005.08.071]
32. Rajaei N, Pirali K, Habibian S, Azizi H. Evaluation of lethal effect of aqueous and hydroalcoholic extracts of Achillea talagonica on the Emeria maxima oocysts in vitro. Iran J Vet Clin Sci. 2017;11(1):15-26. [Persian] [Link]
33. Bahri Najafi R, Motazedian M, Azadbakht M, Sodagar R. Effect of essential oils from some medicinal plants on giardia lambelia cyst in comparison with metronidazol. Esfehan Univ Res J. 2003;17(2):199-206. [Link]
34. Chen M, Christensen SB, Blom J, Lemmich E, Nadelmann L, Fich K, et al. Licochalcone A, a novel antiparasitic agent with potent activity against human pathogenic protozoan species of Leishmania. Antimicrob Agents Chemother. 1993;37(12):2550-6. [Link] [DOI:10.1128/AAC.37.12.2550]
35. Hussain K. Evaluation of anticoccidial and immunomodulatory effects of crude herbal extracts in chicken [Dissertation]. Faisalabad: University of Agriculture; 2016. [Link]
36. Schwikkard S, Van Heerden FR. Antimalarial activity of plant metabolites. Nat Product Rep. 2002;19(6):675-92. [Link] [DOI:10.1039/b008980j]
37. Kim HK, Park Y, Kim HN, Choi BH, Jeong HG, Lee DG, et al. Antimicrobial mechanism of β-glycyrrhetinic acid isolated from licorice, Glycyrrhiza glabra. Biotechnol Lett. 2002;24(22):1899-902. [Link] [DOI:10.1023/A:1020900124997]
38. Fiore C, Eisenhut M, Krausse R, Ragazzi E, Pellati D, Armanini D, et al. Antiviral effects of Glycyrrhiza species. Phytother Res. 2008;22(2):141-8. [Link] [DOI:10.1002/ptr.2295]
39. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T. Anti-Helicobacter pylori flavonoids from licorice extract. Life Sci. 2002;71(12):1449-63. [Link] [DOI:10.1016/S0024-3205(02)01864-7]
40. Giannenas I, Florou-Paneri PC, Papazahariadou M, Christaki E. Dietary oregano essential oil supplementation on performance of broilers challenged with Eimeria tenella. Arch Anim Nutr. 2003;57(2):99-106. [Link] [DOI:10.1080/0003942031000107299]
41. Allen PC. Dietary supplementation with Echinacea and development of immunity to challenge infection with coccidia. Parasitol Res. 2003;91(1):74-8. [Link] [DOI:10.1007/s00436-003-0938-y]
42. Allen PC, Danforth HD, Augustine PC. Dietary modulation of avian coccidiosis. Int J Parasitol. 1998;28(7):1131-40. [Link] [DOI:10.1016/S0020-7519(98)00029-0]
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