Volume 22, Issue 4 (2019)
mjms 2019, 22(4): 197-202 |
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Moghadam P, Khadivi Derakhshan F. Isolation and Identification of Halophilic Bacteria Producing Asparaginase Enzyme from Urmia Lake. mjms 2019; 22 (4) :197-202
URL: http://mjms.modares.ac.ir/article-30-38082-en.html
URL: http://mjms.modares.ac.ir/article-30-38082-en.html
1- Department of Microbiology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran
2- Department of Biology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran , khadivi.d.f@gmail.com
2- Department of Biology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran , khadivi.d.f@gmail.com
Abstract: (2348 Views)
Aims: The present study was aimed to investigate isolation and identification of halophil bacteria producing asparaginase from Urmia Lake and the effect of different salt concentrations and temperature on asparaginase production.
Materials & Methods: The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of halotolerant bacteria. Then, the asparaginase production rate was investigated in saline M9. The asparaginase production rate by isolates was investigated in different salt concentrations (10, 20, 30, and 40%) and temperatures (30, 35, 37, 45, and 50°C). Two isolates with a high production rate of asparaginase were stained by gram stain method and were identified by 16S rRNA sequencing.
Findings: In the present study, 57 isolates were obtained which 10 isolates showed asparaginase production. Among these, 2 isolates (A2 and A3) were selected as superior isolates. The isolate A3 has shown the largest halo (54mm) at 35°C and 10% salt concentration. Moreover, the isolate A2 has shown the largest halo (56mm) at 35°C and 40% salt concentration. The 16SrRNA sequencing showed that the A2 isolate was similar 98.9% to the Halomonas elongata strain, and the A3 isolate was similar 100% to the Bacillus aryabhattai strain.
Conclusion: The bacteria isolated from Urmia Lake are potential source of asparaginase enzyme. H. elongate and B. aryabhattai strains isolated from Urmia Lake can be used to production of asparaginase without side effects for cancer patients.
Materials & Methods: The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of halotolerant bacteria. Then, the asparaginase production rate was investigated in saline M9. The asparaginase production rate by isolates was investigated in different salt concentrations (10, 20, 30, and 40%) and temperatures (30, 35, 37, 45, and 50°C). Two isolates with a high production rate of asparaginase were stained by gram stain method and were identified by 16S rRNA sequencing.
Findings: In the present study, 57 isolates were obtained which 10 isolates showed asparaginase production. Among these, 2 isolates (A2 and A3) were selected as superior isolates. The isolate A3 has shown the largest halo (54mm) at 35°C and 10% salt concentration. Moreover, the isolate A2 has shown the largest halo (56mm) at 35°C and 40% salt concentration. The 16SrRNA sequencing showed that the A2 isolate was similar 98.9% to the Halomonas elongata strain, and the A3 isolate was similar 100% to the Bacillus aryabhattai strain.
Conclusion: The bacteria isolated from Urmia Lake are potential source of asparaginase enzyme. H. elongate and B. aryabhattai strains isolated from Urmia Lake can be used to production of asparaginase without side effects for cancer patients.
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