Volume 23, Issue 3 (2020)
mjms 2020, 23(3): 137-142 |
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Ghasemi N, Falsafi S, Amini K. Evaluation the effect of sea cucumber (Holothuria leucospilota) on invA gene expression in Salmonella infantis. mjms 2020; 23 (3) :137-142
URL: http://mjms.modares.ac.ir/article-30-42239-en.html
URL: http://mjms.modares.ac.ir/article-30-42239-en.html
1- Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran
2- Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran , Sarvnaz_falsafi@yahoo.com
3- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran , Sarvnaz_falsafi@yahoo.com
3- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract: (2431 Views)
Introduction: The invA gene plays a critical role in the pathogenicity of Salmonella infantis. Marine organisms, including sea cucumbers due to their effective secondary metabolites, have been identified and studied with compounds with antioxidant, anti-cancer, antibacterial and anti-inflammatory properties. The aim of this study is to determine effect of sea cucumber extraction (Holothuria leucospilota) on S. infantis invA gene expression .
Material & Methods: Poultry meat was sampled. The S. infantis strains containing invA gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively.
Results: Morphological and biochemical characteristics of these bacteria were confirmed. From 450 samples, 12 S.infantis isolates were isolated. The PCR technique was used to identify the invA encoding gene. All 12 isolates have invA virulence genes. MIC was determined 256 µg/ml. The effect of sea cucumber extract on invA gene expression in S.infantis was evaluated, and the rate of change for the invA gene is estimated -1.21.
Discussion & Conclusion: According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against S. infantis
Material & Methods: Poultry meat was sampled. The S. infantis strains containing invA gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively.
Results: Morphological and biochemical characteristics of these bacteria were confirmed. From 450 samples, 12 S.infantis isolates were isolated. The PCR technique was used to identify the invA encoding gene. All 12 isolates have invA virulence genes. MIC was determined 256 µg/ml. The effect of sea cucumber extract on invA gene expression in S.infantis was evaluated, and the rate of change for the invA gene is estimated -1.21.
Discussion & Conclusion: According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against S. infantis
Keywords: Salmonella infantis, Extracts of the sea cucumber, Antimicrobial activity, Gene expression
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