Volume 13, Issue 4 (2011)                   mjms 2011, 13(4): 0-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ayoubian N, Foroutan T, Jamshidifar A, Mosaffa N. The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro. mjms 2011; 13 (4)
URL: http://mjms.modares.ac.ir/article-30-5245-en.html
The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro
Nader Ayoubian1 , Tahereh Foroutan 2, Azin Jamshidifar1 , Nariman Mosaffa3
1- Department of Periodontology, Faculty of Dentistry, Islamic Azad University, Tehran Branch
2- Department of Biology, Faculty of Basic Science, Tarbiat Moalem University, Tehran
3- Professor, Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (7596 Views)
Objective: Osteon has been introduced as a bone substitute material. It is biphasic calcium phosphate (BCP) that use in dentistry. The aim of the present study was to evaluate the effect of osteon on the proliferation, cell viability and differentiation of saos-2 cells in vitro. Also it was compared with cerasorb bone graft. Materials and Methods: Two different bone grafts materials, osteon and cerasorb, were used to evaluate the effect on proliferation and differentiation rate of saos-2 cells. On day 15 the cell proliferation and cell viability was measured by MTT assay. For determination of differentiation, alkaline phosphatase and alizarin red test was used. Results: Osteon and cerasorb groups showed significantly higher alkaline phosphatase activity and cell differentiation. Cell viability of both bone grafts was significantly lower than control group and cell proliferation was higher in osteon group. Osteon has more suitable biological property compare to cerasorb. Conclusions: The results of the present study showed that osteon and cersorb bone grafts allow proliferation and differentiation of saos-2 cells in vitro.
Keywords: Bone Substitute Replacement, Cell Viability, Cell Line Saos-2, Morphology
Full-Text [PDF 1205 kb]   (4358 Downloads)    

Received: 2010/08/14 | Accepted: 2010/12/27
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.