Volume 16, Issue 1 (2013)                   mjms 2013, 16(1): 23-39 | Back to browse issues page

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Tahmasebi Mirgani M, Sadeghizadeh M, Najafi F, Mowla S J. Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells. mjms 2013; 16 (1) :23-39
URL: http://mjms.modares.ac.ir/article-30-5759-en.html
Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in 5637 Bladder Cancer Cells
Maryam Tahmasebi Mirgani1 , Majid Sadeghizadeh 1, Farhood Najafi2 , Seyed Javad Mowla1
1- Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
Abstract:   (8743 Views)
Objective: The anti-cancer properties of curcumin, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. However, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing curcumin's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier called dendrosome. This study intends to evaluate the role of dendrosomal-curcumin (DNC) in bladder cancer cell growth. Methods: We performed the MTT assay, flow cytometry and Annexin V-FLUOS (as an apoptosis detection kit) to evaluate cell death. The genetic mechanism of DNC-induced apoptosis was accomplished by a study of the relative expressions of OCT4A, OCT4B1, SOX-2 and Nanog using real-time PCR. Results: DNC-induced cell death complied with a time and dose-dependent paradigm in the 5637 cell line. Cell cycle analysis revealed that the number of cells increased in pre-G1 and gradually decreased in G1 and S phases. This showed the inhibitory property of dendrosomal-curcumin on DNA synthesis. Data from real-time PCR determined that expressions of OCT4A, OCT4B1, SOX-2 and Nanog could be related to 5637 cancer cell growth. Dendrosomal-curcumin significantly suppressed mRNA expression of the above mentioned genes (pConclusion: The data showed that DNC induced apoptosis by suppression of pluripotency genes in 5637 bladder cancer cells, which confirmed the useful characteristic of nano-drug in bladder cancer therapy
Keywords: Bladder cancer, Dendrosome, Curcumin, Cell-programmed death, Pluripotency genes
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Article Type: Original Manuscipt | Subject: Molecular Genetics|Molecular Biology|Biotechnology
Received: 2013/01/29 | Accepted: 2013/05/19
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