Volume 14, Issue 3 (2011)
mjms 2011, 14(3): 1-13 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Izadi P, Noruzinia M, Karimipour M, Karbassian M H, Akbari M T, Fereidooni F, et al . Study of the association between estrogen receptor alpha gene expression in the levels of RNA and protein in primary breast tumors. mjms 2011; 14 (3) :1-13
URL: http://mjms.modares.ac.ir/article-30-9439-en.html
URL: http://mjms.modares.ac.ir/article-30-9439-en.html
Pantea Izadi1 
, Mehrdad Noruzinia 2, Morteza Karimipour3 , Mohammad Hamid Karbassian4 , Mohammad Taghi Akbari2 , Forouzandeh Fereidooni5 , Zahra Mostakhdemin Hosseini5 , Fatemeh Kamali6 , Issa Jahanzad5
, Mehrdad Noruzinia 2, Morteza Karimipour3 , Mohammad Hamid Karbassian4 , Mohammad Taghi Akbari2 , Forouzandeh Fereidooni5 , Zahra Mostakhdemin Hosseini5 , Fatemeh Kamali6 , Issa Jahanzad5
1- Ph.D. Student, Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Assistant Professor, Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Assistant Professor, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
4- Breast Surgeon, Day Hospital, Tehran, Iran
5- Pathologist, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
6- G.P. Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
2- Assistant Professor, Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Assistant Professor, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
4- Breast Surgeon, Day Hospital, Tehran, Iran
5- Pathologist, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
6- G.P. Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
Abstract: (5257 Views)
Objective: Estrogen receptor alpha protein status is determined by routine immunohistochemistry analysis in all malignant breast tumors. This assay has its limitations. RNA based techniques are potential complements for immunohistochemistry but it must be noticed that gene silencing may occur at different levels from RNA to protein. The aim of this study was the comparison of the results from these two assays and characterizing the tumors subgroup in which gene expression occurs at RNA level but the target protein is absent.
Materials and Methods: 92 primary breast tumors including their clinical and IHC results were collected before treatment. Estrogen receptor gene expression of tumors was studied by Reverse Transcription Polymerase Chain Reaction (RT PCR). In this assay, GAPDH was used as a reference gene.
Results: 36.6 % of tumors with negative estrogen receptor protein showed gene expression at mRNA level. In this subgroup most of the patient were older than 50 years and in stages 3 or 4 of breast cancer and had poor prognosis according to Nottingham prognostic index. Most cases of the perineural invasion have been seen in this subgroup.
Conclusion: It seems that RT-PCR assay would enable us to recognize a subgroup of breast tumors with poor prognosis which expresses RNA but not protein.
Article Type: Original Manuscipt |
Subject:
Medical Genetics
Received: 2011/04/23 | Accepted: 2011/09/27
Received: 2011/04/23 | Accepted: 2011/09/27
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |