Volume 15, Issue 1 (2012)                   mjms 2012, 15(1): 81-91 | Back to browse issues page

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Yazdani L, Ravanshad M, Khanlari Z, Yaghoobi R. Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients. mjms 2012; 15 (1) :81-91
URL: http://mjms.modares.ac.ir/article-30-4006-en.html
Application of Semi-nested PCR RFLP for Detection and Genotyping GBV-C among HBV-infected Patients
Laleh Yazdani1 , Mehrdad Ravanshad 1, Zahra Khanlari1 , Ramin Yaghoobi2
1- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (6124 Views)
Objective: GBV-C is a virus transmissible via blood transfusion and sexual routes. Because of the shared transmission routes in GBV-C and hepatitis B virus (HBV) we have attempted to detect GBV-C in HBsAg-positive patients using the more sensitive semi-nested PCR. We used restriction fragment length polymorphism (RFLP) to determine genotype. Methods: A total of 100 serum samples were collected from HBsAg-positive patients from 1389-1390. After designing specific primers and optimizing the semi-nested PCR, sequences of PCR products were analyzed by Neb Cutter software. Restriction enzyme sites were determined and suitable enzymes selected for RFLP. Results: Semi-nested PCR shows acceptable sensitivity for the detection of GBV-C and its genotype determination. This technique is more affordable than other techniques. Conclusion: There appears to be a high rate of GBV-C infection among Iranian HBV patients. In this study, the majority of patients co-infected with GBV-C were of HBV genotype 2, which is similar to the pattern seen in the US and Europe.
Keywords: RFLP, Semi Nested PCR, HBV, RFLP, Genotyping, GBV-C, HBs-Ag Positive, Semi-Nested PCR
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Article Type: Original Manuscipt | Subject: Virology
Received: 2011/10/1 | Accepted: 2011/12/27
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