Volume 13, Issue 4 (2011)
mjms 2011, 13(4): 0-0 |
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Rabbani M, Tafazoli Shadpour M, Shokrgozar M A, Janmaleki M, Amirizade N, Haghighipour N. Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells. mjms 2011; 13 (4)
URL: http://mjms.modares.ac.ir/article-30-8813-en.html
URL: http://mjms.modares.ac.ir/article-30-8813-en.html
Mohsen Rabbani 
, Mohammad Tafazoli Shadpour 1, Mohammad Ali Shokrgozar2 , Mohsen Janmaleki3 , Naser Amirizade4 , Nooshin Haghighipour2
, Mohammad Tafazoli Shadpour 1, Mohammad Ali Shokrgozar2 , Mohsen Janmaleki3 , Naser Amirizade4 , Nooshin Haghighipour2
1- Department of Biomechanics, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran
2- National Cell Bank of Iran, Pasteur Institute of Iran, Tehran
3- Nanomedicine and Tissue Engineering Research Center, Taleghani Hospital, Parvaneh St., Velenjak
4- Assistant Professor, Department of Hematology, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
2- National Cell Bank of Iran, Pasteur Institute of Iran, Tehran
3- Nanomedicine and Tissue Engineering Research Center, Taleghani Hospital, Parvaneh St., Velenjak
4- Assistant Professor, Department of Hematology, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
Abstract: (10166 Views)
Objective: Cell vital function has correlation with mechanical loadings that cell experiences. Here, effects of in-vitro combined cyclic-static stretch on proliferation of human mesenchymal stem cell (HMSC) were evaluated.
Materials and Methods: HMSCs were cultured on gelatin coated elastic membranes, and exposed to stretch loading. Four different regimes of cyclic, static, combined cyclic-static, and cyclic with a period of unloading were exerted on the elastic membrane. Duration of cyclic loading and static loading was 5 and 12 hours respectively.
Results: The results illustrate that 10% cyclic stretch causes cell alignment but there were no significant proliferation differences between control and test group. Combined cyclic-static stretch reduced proliferation significantly while cyclic stretch with an unloading period increased cell proliferation significantly. At last, static stretch did not affect cell proliferation significantly.
Conclusion: Cell stretching regimes and post-loading duration are effective factors on cell proliferation.
Keywords: Tissue engineering, Static Stretch, Cyclic Stretch, Cell Proliferation, Human Mesenchymal Stem Cell
Received: 2010/09/14 | Accepted: 2010/12/11
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