Tarbiat Modares University Pathobiology Research 2538-3000 12 4 2010 10 01 Detection of murine tissues that express opticin شناسایی بافت‏های موشی بیان‏کننده پروتئین Opticin 31 38 19177 EN Zahra Tahmasebi Fard Assistant Professor, Department of Biology, Islamic Azad University‮ of Roudehen Branch, Tehran, Iran Kazem Parivar Professor, Department of Developmental Biology, Islamic Azad University, Science and Research Unit, Tehran, Iran Leila Barzegar Yarmohammadi M.Sc., Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran Mohammad Mehdi Akhondi Associated Professor, Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran Mahmoud Jeddi Tehrani Associated Professor, Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran Hojjatollah Rabbani Associated Professor, Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran Journal Article Objective: The LRR (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, DNA repair and RNA processing. Opticin is a member of this family. Takanosu et al (2001) detected messenger RNA expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization. In this study, expression levels of mRNA and protein of opticin was investigated by two monoclonal antibodies which were raised against opticin peptides. By this method structure of opticin in human and mouse has been studied. Materials and Methods: Mouse tissues including, kidney, testis, liver, lung, heart, brain, muscle, spleen and eye were isolated. Opticin expression was identified at mRNA and protein levels by RT-PCR and Western blot. Results: PCR analysis revealed that opticin mRNA is expressed in all the tissues studied except for the lung. However, opticin protein was detected in all tissues analyzed. Conclusion: Expression of opticin in the adult murine tissues may suggest functions other than that of putative regulation of vitreous collagen fibrillogenesis for this molecule. https://mjms.modares.ac.ir/article_19177_7952f28ed57f184d368a7d2a47947cd7.pdf