Tarbiat Modares University Pathobiology Research 2538-3000 20 3 2017 10 01 Production of embryonic stem like-cells from neonatal mouse testis after exposure to low-intensity ultrasound ایجاد سلول‌های بنیادی شبه جنینی از بیضه موش نوزاد پس از تابش امواج فراصوت با شدت پایین 49 60 19308 EN Arian Azimi Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zohreh Mazaheri Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zohreh Mazaheri Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mansoreh Movahedin Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Journal Article Objective: Pluripotent stem cells derived from testis are a new, unlimited source for cell therapy in regenerative medicine. Recently, studies show that spermatogonial stem cells can form embryonic stem-like cells (ES-like cells) in vitro. New procedures such as low intensity ultrasound (LIUS) can have positive effects on cell growth and differentiation. However, the effect of LIUS stimulation on ES-like cells has not been explored. In this study we investigate the effects of LIUS on colonization of ES-like cells. Methods: Initially, we isolated SSCs from neonatal mice. The spermatogonial and Sertoli cells were cultured together in DMEM/F12 supplemented with 15% fetal bovine serum (FBS) and leukemia inhibitory factor (LIF). ES-like cells were stimulated by LIUS at intensity doses of 200 millwatt/square centimeter (mW/cm2) over 5 days. Characteristics of the isolated cells were confirmed by immunocytochemistry with Sox2 and SSEA-1 protein for ES-like cells. We also investigated colonization features in the ES-like cells. Results: After 21 days, we observed there was a significant increase in diameter and number of colonies in the 200 mW/cm2 group compared to the control group (p≤0.05). Pluripotency proteins, ES-like cell marker Sox2, and SSEA-1 expressed in the ES-like cells. Conclusion: LIUS treatment can be an efficient, cost-effective method to improve colonization of ES-like cells during culture. https://mjms.modares.ac.ir/article_19308_caec785d9e8e4f690ce7d9a4d37cb677.pdf