Tarbiat Modares University Pathobiology Research 2538-3000 21 2 2018 07 01 Preparation and Characterization of Smart Poly (N-‎Isopropylacrylamide)‎‌ ‌Nanoparticles Containing Vascular ‎Endothelial Growth Factor for Induction of Angiogenesis in ‎Human Bone Marrow-derived Mesenchymal Stem Cells ‎ ساخت و مشخصه‌یابی نانوذرات هوشمند ‌پلی‌ان‌ایزوپروپیل‌اکریل‌آمید حاوی فاکتور ‌رشد‎ ‎اندوتلیال عروقی برای القای رگ‌زایی در ‌سلول‌های بنیادی مزانشیمی مشتق از مغز ‌استخوان انسانی 65 72 19322 EN A. Adibfar Life Science Engineering Department, New Sciences & Technologies Faculty, University of Tehran, Tehran, ‎Iran Gh. Amoabediny ‎ Chemical Engineering Departmen, Chemical Engineering Faculty, University of Tehran, Tehran, Iran M.R. Baghaban Eslaminejad ‎ Stem Cells & Developmental Biology Department, Cell Science Research Center, Royan Institute for Stem Cell ‎Biology & Technology, Tehran, Iran Fatemeh. Bagheri Biotechnology Department, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, Iran B. Zandieh Doulabi Oral Cell Biology Department, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam & ‎VU University, Amsterdam, The Netherlands J. Mohamadi ‎ Life Science Engineering Department, New Sciences & Technologies Faculty, University of Tehran, Tehran, ‎Iran Journal Article <strong>Aims: </strong>Growth factor (GFs) delivery with the certain concentration and release kinetic is one of the main challenges in tissue engineering. The aim of this study was the preparation and characterization of smart poly (N-isopropylacrylamide) nanoparticles containing vascular endothelial growth factor for induction of angiogenesis in human bone marrow-derived mesenchymal stem cells.<br> <strong>Materials and Methods</strong>: In this exprimental study, two different formulations of temperature-sensitive Poly (N-isopropylacrylamide) (PNIPAM) nanoparticles (NPs) were synthesized by free radical polymerization technique. Nanoprecipitation and diffusion methods were used to load the vascular endothelial growth factor (<em>VEGF</em>) in PNIPAM NPs. The effects of released VEGF on the differentiation of human bone marrow stem cells (hBMSCs) into endothelial cells in angiogenic, osteogenic, and 50% angiogenic-osteogenic culture medium were investigated, using flow cytometry and light microscope. Statistical analysis was performed, using the GraphPad Prism 6 software.<br> <strong>Findings:</strong> The nanoprecipitation process caused polymer degradation due to using the organic <em>N, N</em>-<em>Dimethylacetamide </em>solvent. The cumulative VEGF released after 72hours for 70%. A total of 10ng/ml VEGF released from PNIPAM nanoparticles, in 2D culture with cell density of 3×10⁴hBMSCs, after 7 days, leading to the endothelial differentiation, capillary-like tube formation, and expression of 20% vWF as angiogenic marker.<strong><span dir="RTL"></span></strong><br> <strong>Conclusion:</strong> The PNIPAM NPs have the potential to load and release the angiogenic GFs for induction of angiogenesis in hBMSCs and in osteogenic medium. https://mjms.modares.ac.ir/article_19322_4a7d2be8648d00c3d1090ed4168a5748.pdf