Tarbiat Modares University Pathobiology Research 2538-3000 21 3 2018 10 01 Anti-angiogenesis Effect of C-Phycocyanin of Spirulina platensis on B16-F10 Melanoma Tumors in C57BL/6 Mouse اثر ضدرگ‌زایی فیکوسیانین سی اسپیرولینا پلاتنسیس بر رده سرطانی ملانومای B16-F10 در موش سوری C57BL/6 141 146 19332 EN F. Dibaei Biology Department, Basic Sciences Faculty, Isfahan Branch, Payam-e-Noor University, Isfahan, Iran M. Fazilati Biology Department, Basic Sciences Faculty, Isfahan Branch, Payam-e-Noor University, Isfahan, Iran F. Moenzadeh Pathology Department, Medicine Faculty, Najaf Abad Branch, Islamic Azad University, Isfahan, Iran A.H. Kafayat Cancer Research Center, Isfahan University of Medical Sciences, Isfahan, Iran N. Jazayeri Biology Department, Basic Sciences Faculty, Isfahan Branch, Payam-e-Noor University, Isfahan, Iran A. Talebi Pathology Department, Medicine Faculty, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article <strong>Aims:</strong> Melanoma is one of the most dangerous forms of skin cancer, which is unresponsive to the current chemotherapy drugs. As a natural product purified from <em>spirulina</em>, phycocyanin can inhibit the angiogenesis. The aim of this study was to investigate the anti-angiogenesis effect of C-phycocyanin of <em>spirulina platensis</em> on B16-F10 melanoma tumors in C57BL/6 mouse.<br> <strong>Materials and Methods:</strong> In this experimental study, 16 C57BL/6 mice with the age range of 6-8 weeks were randomly divided into two groups, including control and phycocyanin groups. On the day 0 of the study, melanoma cells were injected and all the mice were treated for 20 days. Phycocyanin group received 40mg/kg phycocyanin every day. The tumors were extracted on the day 21 and the effect of phycocyanin on the angiogenesis and proliferation of cancer cells was investigated, using immunohistochemical staining with CD31 and Ki-67, respectively. The data were analyzed, using JMP 11 software by one way ANOVA test.<br> <strong>Findings: </strong>In the phycocyanin group, angiogenesis was significantly lower than that of the control group (p<0.01), while the mitotic index was not significantly lower than that of the control group in the mice treated with phycocyanin.<br> <strong>Conclusion:</strong> Phycocyanin has ability to inhibit angiogenesis in the B16-F10 melanoma tumors in C57BL/6 mouse, but it is not able to reduce the proliferation of melanoma cells.<br> https://mjms.modares.ac.ir/article_19332_f182c4c2e7b4bd91debd2d0d636becac.pdf