Tarbiat Modares University Pathobiology Research 2538-3000 23 3 2020 07 01 Evaluation the effect of sea cucumber (Holothuria leucospilota) on invA gene expression in Salmonella infantis بررسی اثر عصاره خیار دریایی (Holothuria leucospilota) بر بیان ژن invA در سالمونلا اینفنتیس 137 142 19380 EN N. Ghasemi Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran S. Falsafi Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran K. Amini Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran Journal Article <strong>Introduction:</strong> The <em>invA</em> gene plays a critical role in the pathogenicity of Salmonella infantis. Marine organisms, including sea cucumbers due to their effective secondary metabolites, have been identified and studied with compounds with antioxidant, anti-cancer, antibacterial and anti-inflammatory properties<strong><span dir="RTL">. </span></strong>The aim of this study is to determine effect of sea cucumber extraction (Holothuria leucospilota) on <em>S. infantis</em> <em>invA</em> gene expression <span dir="RTL">.</span><br> <strong>Material & Methods:</strong> Poultry meat was sampled. The <em>S. infantis </em>strains containing <em>invA</em> gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively.<br> <strong>Results:</strong> Morphological and biochemical characteristics of these bacteria were confirmed. From 450 samples, 12 S.<em>infantis</em> isolates were isolated. The PCR technique was used to identify the <em>invA</em> encoding gene. All 12 isolates have <em>invA</em> virulence genes. MIC was determined 256 µg/ml. The effect of sea cucumber extract on invA gene expression in S.<em>infantis</em> was evaluated, and the rate of change for the <em>invA</em> gene is estimated -1.21.<br> <strong>Discussion & Conclusion:</strong> According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against <em>S. infantis</em> https://mjms.modares.ac.ir/article_19380_0b7fd9d2155471c9ae714ee8b6709386.pdf