Tarbiat Modares University Pathobiology Research 2538-3000 25 3 2022 04 01 Effect of deep brain stimulation on length of dendrites in hippocampal CA1 neurons in pentylenetetrazol kindled rats 26 31 19451 EN Razieh Rohani Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abbas Aghaei Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad-Amin Abdollahifar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hamid Reza Mirbehbehani Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Hassan Heidari Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article <span>Aim:</span><span> Applying deep brain stimulation at low-frequency has anticonvulsant effect on kindled seizures. In this study, the effect of low-frequency stimulation (LFS) on kindling-induced changes in the dendritic length of hippocampal CA1 neurons was investigated. </span><br> <span>Methods:</span><span> To administer LFS in the hippocampus, animals first underwent stereotactic surgery and a tripolar electrode were positioned in the CA1 region. Animals received intraperitoneal pentylenetetrazole (PTZ; 34 mg/kg) every other day until they showed three consecutive stage 4 or 5 seizures. LFS was administered to the dorsal hippocampal CA1 area in kindled+LFS group. Hippocampal samples were prepared for stereological assessment one week after termination of LFS application.</span><br> <span>Results: </span><span>PTZ kindling was accompanied with a decrease in dendritic length in CA1 neurons. One week after application of LFS, the length of dendrites was restored to control group values, and there was a significant difference between kindled+LFS and kindled groups. Interestingly, the effect of administering LFS alone in control group, was similar to that of kindled group and a significant decrease was observed in dendritic length.</span><span> <span dir="RTL"></span><br> <span>Conclusion: </span><span>LFS had a restoring effect on morphological changes in CA1 neurons of kindled animals. This effect may be considered a mechanism for therapeutic action of deep brain stimulation in seizure.</span> https://mjms.modares.ac.ir/article_19451_ea9c07b5d0be2b8ee4631ee110f97fb4.pdf