Tarbiat Modares University Pathobiology Research 2538-3000 26 4 2023 07 01 The effect of adherent and non-adherent culture plates on the detachment of PMA-treated THP-1 cell line 27 32 19490 EN Mahboobeh Ebrahimi Varkiani Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Sadaf Vahdat Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Journal Article <span>Introduction: </span><br> <span>Due to their role in regulating inflammation, monocytes and macrophages are important immune system cells incorporated and evaluated in various in vitro and in vivo disease modeling experiments. Accordingly, investigating appropriate culture conditions to maintain the viability, phenotype, and functionality of these cells is considered in different studies. In this study, we tried to evaluate whether the type of culture plate affects the adhesion, survival, and morphology of PMA-treated monocytes.</span><br> <span>Methods:</span><br> <span> <span>The THP-1 cell line was cultured in adherent or non-adherent culture plates and cells were treated with PMA small molecule to be induced into macrophages. The morphology of treated cells and the viability of detached cells were assessed three days post-induction. </span><br> <span>Results and conclusion:</span><br> <span>Our results showed that the morphology and viability of PMA-treated THP-1 cells were the same in both types of plates. In summary, we showed that the type of cell culture plate did not significantly affect PMA-treated THP-1 cells·</span> https://mjms.modares.ac.ir/article_19490_db35b1f3d006b037c6acf898da036d58.pdf