Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Unveiling a specific immunogen protein in A. baumannii رونمایی یک پروتئین ایمونوژن اختصاصی در اسینتوباکتر بومانی 1 16 19256 EN Kobra Ahmadi Zanoos Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Iraj Rasooli Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Abolfazl Jahangiri Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Reza Rahbar Department of Molecular Biology, Dr. Saadati’s Laboratory, Shiraz, Iran Shakiba Darvish Alipour Astaneh Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Journal Article Objectives: Acinetobacter baumannii is a Gram-negative, non-motile aerobic bacterium known as a nosocomial pathogen resisting often to broad range of antibiotics. The pathogen is a serious agent of mortality and morbidity in hospital particularly among immunocompromise patients. Treatment and control of its infections is complicated owing to its high antibiotic resistance, survival in various environmental conditions and utilization of wide range of nutrient sources. Early detection of the pathogen in established infections is pivotal for controlling the infections. Culture and biochemical test are current methods for detection of the bacterium taking 2-5 days time. Hence, a new rapid specific affordable diagnostic test is needed. Development of such test depends on a suitable biomarker without any cross reactivity with other bacteria. Methods: Accordingly, aim of the study is to unveil of a 34.4 KDa outer membrane protein (OMP) introduced by Islam et al. in A. baumannii ATCC19606. In the current study, we harnessed various bioinformatic servers to screen whole proteome of the bacterium. Properties critical to the screening include: molecular weight, localization, topology, homology, antigenicity and allergenicity of proteins. Results: Three proteins were found as suitable candidate molecular weight as well as localization points of view. BLAST searches, antigen probability predictions and other analyses led to selection of one protein as the best specific antigen of A. baumannii. Conclusion: The in silico analyses unveiled the best candidate protein vide accession number ZP_05827218.1.

https://mjms.modares.ac.ir/article_19256_b61557c54dfb0ba8eaae27760bd98c63.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Investigation of Tumorigenicity ability of Immature Male Mouse Spermatogonial Stem Cells after In Vitro Cultivation and Inoculation in Athymic Animals سنجش تومورزایی سلول‏های بنیادی زایای موش مذکر نابالغ تکثیر یافته در محیط کشت پس از پیوند به موش بالغ با نقص سیستم ایمنی 17 27 19257 EN Zohreh Mazaheri Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mahnaz Haddadi Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Zohreh Mazaheri Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Saeid Amanpour Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Samad Muhammadnejad Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Ahad Muhammadnejad Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Mansoureh Movahedin Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran Journal Article Objective: It is hypothesized that stem cells have the capability to form tumors after transplantation. Spermatogonial stem cells have proliferation potency and colonization ability related to express pluripotency genes such as c-Myc. The primary aim of this study is to investigate tumorigenicity ability of these cells after in vitro cultivation and inoculation in athymic animals. Methods: Spermatogonial stem cells from 3-5 day-old neonatal mice testes (NMRI) were cultured following two-step enzymatic digestion. After one month of culturing the spermatogonial stem cells, the obtained colonies were identified by Oct4 and PLZF markers. Expressions of Nanog, Oct4 and c-Myc pluripotency genes were subsequently studied. We subcutaneously inoculated 5 x 106 cells into athymic mice and assessed tumor formation after 8 weeks. Mouse embryonic stem cells (CCE line) were used as the positive control. Generated tumors were measured by a caliper. Results: The colonies expressed Oct4 and PLZF proteins. Ratio of pluripotency gene expressions in these cells compared to embryonic stem cells significantly decreased (P≤0.05). Mouse embryonic stem cells formed tumors however the spermatogonial colonies did not form any tumors. Conclusion: Mouse spermatogonial stem cells in comparison with embryonic stem cells are not capable of forming tumors in vivo. We have observed that the tumorigenic ability of these cells decreased significantly with down regulation of pluripotency gene expressions, particularly c-Myc. However, this study should be reassessed by using human tissue samples.

https://mjms.modares.ac.ir/article_19257_8f7d4d570dd8d2de4e4713dc37719db8.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Rapid Screening for Synthetic Adulterant Drugs in Herbal Slimming Products غربالگری سریع مواد ترکیبی غیر مجاز در داروهای لاغری گیاهی 29 38 19258 EN Marjan Khazan Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mehdi Hedayati Cellular & Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Farzad Kobarfard Department of Medical Chemistry, School of Pharmacy, Shahid Beheshti University, Tehran, Iran Sahar Askari Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Fereidoun Azizi Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article Objective: In recent years the use of herbal slimming supplements has increased in Iran. One problem may be the illegal inclusion of synthetic drugs by some manufacturers in these products. The aim of this study is to determine four undeclared synthetic adulterants in some herbal slimming products present in the Iranian market. Methods: This survey studyresearched six common herbal slimming supplements that were obtained from the market. Supplements were purchased from Persian language advertising sites on satellite channels and the Internet. These products were analyzed by GC-MS for the detection of sibutramine, phenolphthalein, phenytoin and LC-MS for bumetanide. Results: Three products contained phenolphthalein. Fast Slim and Original Super Slim carried the highest content of sibutramine - 57 µg per capsule for Fast Slim and 78 µg per capsule for Original Super Slim. Bumetanide was found in Herbaceous Essence, Green Lean Super Slim, Magic Slim and Fat loss. Other undisclosed components such as caffeine and pseudoephedrine were detected by GC-MS library search. Conclusion: All herbal slimming products have been shown to contain undeclared synthetic adulterants. Increased public awareness of the risks of taking herbal weight-loss supplements is necessary.

https://mjms.modares.ac.ir/article_19258_f2ee555e877c9bd7239e2a2d4e5c4f25.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 The let-7f microRNA Functions as a Negative Regulator of HNF4a Expression in Human Adipose Tissue-derived Stem Cells ترانسداکشن لنتی ویروسی میکرو RNAlet-7f منجر به افزایش بیان ژن HNF4a در سلول‏های بنیادی جدا شده از بافت چربی انسانی می‏شود 39 49 19259 EN Nahid Davoodian Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran Abbas Sahebghadam Lotfi Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran Masoud Soleimani Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Seyed Javad Mola Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Journal Article Objective: microRNAs (miRNAs) are noncoding RNAs that function as key regulators of diverse biological activities such as cellular metabolism, cell proliferation and cell cycle regulation. Recent studies have indicated the high potential of these small molecules to control stem cell differentiation into desired cells. The aim of present study is to investigate the possible effect of let-7f on expression of hepatic nuclear factor 4 alpha (HNF4a) and some hepatic specific factors such as albumin (ALB), alpha fetoprotein (AFP), cytokeratin18 (CK18) and cytokeratin19 (CK19) in human adipose tissue derived stem cells (hADSCs). Methods: ADSCs were isolated from human adipose tissue using collagenase type I and were transduced by recombinant lentiviruses that contained human inhibitor let-7f and Scramble (negative control). Afterward, the expressions of HNF4a, ALB, AFP, CK18 and CK19 were evaluated by Real-time PCR at different time points. Results: Transduction efficiency of lentiviral vectors into ADSCs was more than 80% as judged by the expression of the GFP reporter gene. Real-time PCR analysis revealed that inhibition of let-7f in hADSCs resulted in significant up regulation of hepatic specific genes compared with the negative control. The expression level of HNF4a also increased in experimental cells at day 14, which supported the suppression of HNF4a expression by let-7f. Conclusion: The results of this study identified let-7f as a negative regulator of HNF4a expression in hADSCs and increased the expression of hepatocyte specific factors through silencing of let-7f. Therefore, suppression of let-7f could be a considerable tool for hepatic differentiation of hADSCs.

https://mjms.modares.ac.ir/article_19259_dc754039e4ce819027917a58ab573643.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Role of Two-Partner Secretion System dependent FhaB1 and FhaB2 Proteins in Adhesion of Acinetobacter baumannii ATCC19606 to Human Epithelial Cells نقش دو پروتئین FhaB1 و FhaB2 وابسته به سیستم ترشحی دو جزئی در چسبندگی اسنیتوباکتر بومانی ATCC19606 به سلول اپیتلیال انسانی 51 62 19260 EN Shakiba Darvish Alipour Astaneh Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Iraj Rasooli Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Seyed Latif Mousavi Gargari Molecular Microbiology Research Center, Shahed University, Tehran, Iran Journal Article Objective: Acinetobacter baumannii (A. baumannii) has a good potential to colonize on various surfaces. As a virulence factor, adhesion to surfaces is the first step in colonization. The Two-Partner Secretion System (TPS) proteins are key factors for bacterial attachment. The purpose of this study is to identify and study the role of this family of proteins in adhesion of A. baumannii to human epithelial cells. Methods: Gene homologues that encoded the TPS were analyzed by bioinformatics tools and the primers were designed accordingly. The constructs synthesized in the pET22b vector were transferred to BL21(DE3). The transformed cells were named FhaB1 and FhaB2. The protein expression on the cell membrane was studied in addition to bacterial adhesion and biofilm formation by recombinant strains, A. baumannii and E.coli BL21(DE3). Results: Bioinformatic studies showed the bacterial potential of producing two exoproteins (FhaB1 and FhaB2). Expression of the recombinant proteins on the outer membrane was confirmed by Western Blot Analysis and whole cell ELISA. The results revealed an association between the recombinant cells and bacterial adhesion and bioﬁlm formation. FhaB1, FhaB2 and A. baumannii exhibited enhanced adherence to human lung epithelial cells compared to E.coli BL21(DE3) Conclusion: TPS in A.baumannii is of adherence and colonization factors and is one of the bacterial virulence factors.

https://mjms.modares.ac.ir/article_19260_361f984ff0040fbc17be3547788ad9f3.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Design and Synthesis of a Novel Dendrosome and a PEGylated PAMAM Dendrimer Nanocarrier to Improve the Anticancer effect of Turmeric (Curcuma longa) Curcumin طراحی، سنتز و مقایسه نانو حامل دندروزوم جدید و دندریمر PAMAM پگیله شده در بهبود خواص ضد سرطانی کورکومین زردچوبه (Curcuma longa) 63 77 19261 EN Majid Sadeghizadeh Department of Genetic, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Vahid Erfani-Moghadam Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Alireza Nomani Department of Pharmaceutics, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran Farhood Najafi Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran Yaghoub Yazdani Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran Majid Sadeghizadeh Department of Genetic, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Journal Article Objective: In recent decades, the anticancer effect of curcumin has been proven by several studies. Curcumin affects multiple cell signaling pathways and prevents cell proliferation, invasion, metastasis and angiogenesis. However, the aqueous solubility of curcumin and its bioavailability are very low which restricts its anticancer properties. In this research, we have synthesized a monomethoxy poly (ethylene glycol)-Oleate (mPEG-OA) di-block copolymer and used a surface PEGylated poly (amidoamine) (PAMAM) dendrimer to improve bioavailability of curcumin in cancer cells. Methods: Thecritical micelle concentration (CMC) of mPEG-OA, drug loading efficiencies, and cytotoxicity in the human glioblastoma cell line (U87MG) of all the prepared nanodevices were thoroughly investigated. Results: Atomic force microscopy (AFM) and dynamic light scattering (DLS) studies have shown that mPEG-OA have two common nanostructures, micelles and polymerosomes. mPEG-OA micelles had a very low CMC (0.03 g/l). The IC50 of free curcumin (0.01 methanol solution) was 48 μM, curcumin-loaded mPEG-OA was 24 μM , and curcumin-loaded PAMAM dendrimer was 13 μM. Moreover, the PEGylated PAMAM was non-cytotoxic. Conclusion: The results indicated that by using these nanocarriers, the bioavailability of curcumin significantly increased compared to free curcumin. Overall, this research revealed that these curcumin nanocarriers could be considered as appropriate drug delivery systems for curcumin delivery in cancer cells.

https://mjms.modares.ac.ir/article_19261_9220e1b573ac13ab682c3899a3241db1.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 The Effect of One Event of Submaximal Exercise on Plasma Hepcidin Concentrations in Male Runners تأثیر یک جلسه فعالیت ورزشی زیر بیشینه بر غلظت هپسیدین پلاسما در مردان دونده‌ 79 90 19262 EN Afsaneh Afsari Kalashemi Department of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran Afsaneh Shemshaki Department of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran Mehdi Hedayati Cellular and Molecular Research Center, Research Institute for Endocrine and Metabolism Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article Objective: Because of the importance iron metabolism and role of hepcidin in running, the present study investigates the effects of one event of submaximal exercise on plasma hepcidin concentrations in male runners. Methods: For this purpose, we selected eight runners who were athletic team members of Mazandaran University with an average age of 21.7±1.34 years, height of 175.6±5.88 cm, weight of 71.7±11.13 kg and BMI of 23.2±2.95 kg/m2. Subjects ran on the treadmill at 65% heart rate reserves for 60 min and blood samples were taken before, immediately after, and at 3 and 24 h following exercise. Plasma samples were analyzed to determine the concentrations of hepcidin, IL-6, iron and ferritin. The repeated measures ANOVA with post hoc LSD were used to compare the differences between the samples. Results: The results showed significant decrease in plasma hepcidin at 24 h postexercise compared to immediately postexercise (P<0.05), which might be attributed to a significant decrease in plasma concentrations of IL-6 at 3 h postexercise (P<0.05; r=0.794). Although there were no significant differences observed in postexercise plasma iron, a dramatic increase in plasma ferritin was observed immediately, 3 and 24 h postexercise compared to pre-exercise (P<0.05). Conclusions: With regards to the lack of correlation between plasma concentrations of hepcidin and iron (P>0.05), it can be concluded that one event of submaximal exercise does not cause significant differences on plasma hepcidin concentration immediately postexercise and it does not change in regulation of iron metabolism. This observation may be related to exercise duration and intensity.

https://mjms.modares.ac.ir/article_19262_6a7fcebb1365ff225fcd4c5712017153.pdf

Tarbiat Modares University Pathobiology Research 2538-3000 17 1 2014 04 01 Effects of Aerobic Exercise on C-reactive Protein and Lipid Profile in Subclinical Hypothyroidism among Overweight Obese Women تأثیر تمرین هوازی بر پروتئین واکنشگر C و نیمرخ چربی در زنان مبتلا به کم کاری تیرویید تحت بالینی دارای اضافه وزن- چاق 91 102 19263 EN Somayeh Baharloo Department of Exercise Physiology, School of Physical Education and Sport Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran Farzaneh Taghian Department of Exercise Physiology, School of Physical Education and Sport Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran Mehdi Hedayati Cellular and Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article Objective: The goal of the current research was to investigate the effect of aerobic exercise on C-reactive protein (CRP) and lipid profile in overweight-obese women with subclinical hypothyroidism. Methods: This study enrolled 23 women who suffered from subclinical hypothyroidism. Patients' average age was 41.08±6.56 years and BMI was >25 kg/m2. Subjects, following medical screening, were objectively selected and randomly divided into two groups, control (N=13) and experimental (N=10). Initially, patients' height, weight, BMI, waist ratio, and WHR were measured in addition to serum levels of cholesterol, triglycerides, HDL-C, LDL-C, CRP, T4 and TSH. Then, the experimental group participated in aerobic exercise. The control group received no intervention other than follow up. After 12 weeks all variables were remeasured. For intra group comparison of data t-test was used and for group comparison, we used the independent t-test (P≤0. 05). Results: After 12 weeks of symphonic aerobic exercise in the experimental group, body composition that included weight, BMI, waist ratio, WHR, and levels of cholesterol, LDL-C, CRP and TSH were significantly reduced. There was no significant change in triglycerides, however we observed increased T4 and HDL-C levels. In the control group LDL-C and CRP were significantly increased. Conclusion: The reduction of cardiovascular disease through reduction of body composition and low density cholesterol among over weight-obese subclinical hypothyroidism women is possible through weight reduction with aerobic exercises.

https://mjms.modares.ac.ir/article_19263_93e06678bf43969ed7f3b3377605aa8c.pdf