Comparison of RD, L20B and Hep2 cell lines sensitivity to the standard poliovirus and Oral Polio Vaccine virus

Authors
Zahra Shokati Eshkiki
SHOHREH shahmahmoodi
Mohammadreza Eshraghian
Zahra Mehrabi
Talat Mokhtari Azad
Saied Reza Pakzad
Morteza Pirali Hamedani
Hamideh Tabatabaie
Rakhshandeh Nategh
Virology Division, School of Public Health, Tehran University of Medical Sciences
Abstract
Objective: As we are approaching the global eradication of wild poliovirus, laboratory surveillance of poliovirus by the gold standard cell culture method becomes increasingly important. As there is a lot of concern about accurate and sensitive detection of imported wild and Vaccine Derived Polioviruses (VDPVs) in Polio-Free countries, in this study we assessed and compared the sensitivity of the cell lines used in polio laboratory simultaneously to standard poliovirus and Oral Polio Vaccine (OPV) polioviruses, to ensure the accurate detection of circulating and imported polioviruses in the society.
Materials and Methods: Cell sensitivity test was performed according to the World Health Organization (WHO), Polio Laboratory Manual for RD, L20B and Hep2 cell lines using 3 serotypes of standard monovalent and OPV polioviruses. The test was repeated every four passages for all cell lines.
Results: The sensitivity of L20B and Hep2 cell lines for standard poliovirus type 1 and 2 is more than sensitivity for the same types of OPV virus but for poliovirus type 3 it is vice versa. Also RD cell line is more sensitive to all 3 types of OPV virus. In addition, the test showed that increasing the passage number will decrease the sensitivity of all cell lines.
Conclusion: Using RD & L20B cell lines simultaneously (with low passage number) will assure us of sensitivity and accuracy of the cell lines for detection of circulating and imported polioviruses.
Keywords
RD
L20B
Hep2
Cell sensitivity
Poliovirus
Pathobiology Research
Volume 13, Issue 3
Summer 2010
Pages 31-39