Authors
1
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
2
Department of Marine Biology, Faculty of Marine Sciences and Technology, Azad Islamic University, North Tehran Branch Tehran, Iran
3
Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
4
Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Department of Genetics, Kawsar Human Genetics Research Center, Tehran, Iran
5
Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
6
Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, , Iran
7
of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract
Objective: Sea cucumber is a traditional food and medical item and has been reported to exhibit Antioxidant, antifungal, anti tumoral and antibacterial bioactivity. The objective of this study is to describe the antibacterial activity of 4 extracts of Holoturia. Sp (sea cucumber), collected from Hengam Island of Persian Gulf.
Materials and Methods: Methanol, hexane, aqueous and chloroform extracts from body wall tissue of the sea cucumber were screened for antibacterial activity against three strains of Escherichia coli Top 10 F´, TG1 and K12 using disc diffusion and broth microdilution tests methods.
Result: The growth of all these strains were inhibited using concentration from 0.78 to 100 mg/ml of methanol, hexan and chloroform extracts. Among the extracts just methanol and chloroform with 100 mg/ml had bactericidal effect on TG1 and K12 strains. On the other hand, Aqueous extract had induced growth in of the all strains.
Conclusion: The results suggest the possibility of applying sea cucumber as source of potential anti bacterial agents, whose compounds can be good candidates to make antibiotic products.
Keywords
Pathobiology Reserach