Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation

Authors
Jamshid Hadjati 1
افشین آماری
AliReza Razavi 2
Arezoo Jamali 3
AbbasAli Amini Sardrod 4
Masomeh Motamedi 2
Saeedeh Shojaee 5
Bita Ansaripour 2
1 Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Immunology, Faculty of Paramedicene , Tehran University of Medical Sciences, Tehran, Iran
4 Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Parasithology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Objective: The aim of the present study was to investigate the effect of protein and DNA components of Toxoplasma gondii on maturation of dendritic cells and their efficiency in IL-12 production and proliferation of T cells.
Materials and Methods: for DC generation, Bone marrow cells were cultured in the presence of GM-CSF and IL-4 for 5 days. Tumor lysate and protein or DNA components of Toxoplasma gondii were added to the culture media and incubated for another 2 days. LPS was added as control for DC maturation. Proliferation of T cells were determined by MLR and IL-12 production was measured by ELISA kit. Maturation of dendritic cell were determined by flowcytometry.
Results: DCs treatment with protein components of Toxoplasma gondii caused a significant increase in IL-12 production and proliferation of T cells (P<0.001).
Conclusions: Different compositions of microbial body like protein and DNA components of Toxoplasma gondii can cause augmentation of antigen presentation capacity of DC and their IL-12 production capability. Among these components the protein was more effective as compared to DNA.
Keywords
IL-12
Dendritic cells
Toxoplasma gondii
Pathobiology Research
Volume 12, Issue 3
Summer 2009
Pages 1-8