The Effect of a Co-culture of Mouse Fallopian Tube Cells on Proliferation of Human Endometrial Stem Cells

Authors
S. Ehsani 1
M. Salehnia 1
N. Ghorbanmehr 2
1 Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract
Objective: This study evaluates the effect of a mouse fallopian tube cell co-culture on the proliferation of human endometrial stromal cells.
Methods: We used hysteroscopy to collect the endometrial cells from the uterus. The cells were isolated with collagenase type 3 and passed through 150 µm and 40 µm filters, respectively, after which the isolated cells were cultured in DMEM/F12 medium. At the end of the fourth subculture we used flow cytometry to evaluate the percentage of CD90 positive cells. The endometrial cells were co-cultured with mitomycin C-treated cells from the mouse fallopian tube as the experimental group. Those cultured without the feeder layer were considered the control group. The proliferation rate of cells in both groups were compared by the MTT assay
Results: The endometrial cells adhered to the floor of the plate after 24 hours. After 72 hours, they had a spindle shape which was similar to fibroblasts. The rate of CD90 positive cells at the fourth passage was 94.26 ± 0.08%. The proliferation rate of the co-culture experimental group was 1.1 ± 0.02 and for the control group, it was 1.17 ± 0.17 which was not significantly different.
Conclusion: Co-culture of mouse fallopian tube cells with endometrial stem cells did not affect the proliferation rate of endometrial stem cells.

Keywords

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Pathobiology Reserach
Volume 18, Issue 2
June 2015
Pages 1-11