Combined Effect of Swimming Training and Arbutin Supplementation on Kidney Total Oxidant and Antioxidant Status in Alloxan-induced Diabetic Rats

Authors
M. Zolfalipor 1
P. Farzanegi 1
M. Habibian 2
1 Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran
2 Department of Exercise Physiology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran
Abstract
Objective: Diabetes is a common metabolic disorder that a one of the important factors in the etiology is considered to oxidative damage. The present study intends to study the effect of swimming training with Arbutin on total oxidant status (TOS) and total antioxidant status (TAS) in kidney tissue of Alloxan-induced diabetic rats.
Methods: We randomly divided 42 male Wistar rats that had an average weight of 195 g to 220 g into 6 groups (7 rats per group) - control, diabetic, arbutin, diabetic-arbutin, diabetic-swimming training, and diabetic-combinatorial. Swimming training protocol consisted of 5 days/week for 6 weeks at 5-36 min/day. Diabetes was induced with alloxan [90 mg/kg, intraperitoneal (ip)] in the rats. Arbutin (50 mg/kg, subcutaneous) was administered for 5 days/week. The rats were killed 48 hours after the last treatments and kidney TOS and TAS levels were evaluated. One-way analysis of variance was used for data analysis.
Results: After six weeks of supplementation with arbutin, swimming training and the combination of swimming training and arbutin, we observed a significant decrease in TOS (P<0.05) and elevated TAS (P<0.05) levels in the alloxan-induced diabetic rats.
Conclusion: The combined effect of swimming training and arbutin supplementation can play a major role against renal oxidative stress by modulating total oxidant and antioxidant status in alloxan-induced diabetic rats.

Keywords

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Pathobiology Reserach
Volume 18, Issue 2
June 2015
Pages 85-95