The effect of 4 weeks of endurance exercise on the expression of the muscle Myonectin levels and Insulin resistance in the adult rat

Authors
Elham Vosadi 1
Ali Asghar Ravasi 1
Rahman Soori 1
Zohreh Mazaheri 2
Zohreh Mazaheri 2
Fatemeh Shabkhiz 1
1 Department of Exercise Physiology, Faculty of Physical Education Tehran University, Tehran, Iran
2 Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Purpose of the study: Myokines derived from skeletal muscle modulate metabolic, inflammatory, and other processes action. Myonectin, a novel myokine, expression and Circulating its level were tightly regulated by the metabolic state, fasting suppressed and exercise. The aim of this study was to investigate the effect of 4 weeks of endurance training on gene expression Myonectin muscle activity and plasma insulin resistance, in the male adult rats.
Materials and methods: Sixteen Wistar rats (mean weight: 230 ± 15 g; age: 8 weeks) were divided into two groups: (1) Endurance Training and (2) Control groups. Animals in exercise groups received 4-wk endurance training (5 sessions per week) that included running on a treadmill for 45 minutes and at the same time, the control group did not have any exercise. The Soleus muscle homogenates and the expression of Myonectin genes were measured by Real- time PCR analysis. The enzyme-linked immunosorbent assay (ELISA) method for measuring insulin resistance. The data were analyzed by Independent T Test. Statistical differences were considered significant at P
Keywords
Myonectin
Insulin resistance
Endurance exercise
Adult rat
Pathobiology Research
Volume 19, Issue 2
Summer 2016
Pages 89-97