Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells

Authors
Mohsen Rabbani
Mohammad Tafazoli Shadpour 1
Mohammad Ali Shokrgozar 2
حبیب اله پیروی
Mohsen Janmaleki 3
Naser Amirizade 4
Nooshin Haghighipour 2
1 Department of Biomechanics, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran
2 National Cell Bank of Iran, Pasteur Institute of Iran, Tehran
3 Nanomedicine and Tissue Engineering Research Center, Taleghani Hospital, Parvaneh St., Velenjak
4 Assistant Professor, Department of Hematology, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
Abstract
Objective: Cell vital function has correlation with mechanical loadings that cell experiences. Here, effects of in-vitro combined cyclic-static stretch on proliferation of human mesenchymal stem cell (HMSC) were evaluated.
Materials and Methods: HMSCs were cultured on gelatin coated elastic membranes, and exposed to stretch loading. Four different regimes of cyclic, static, combined cyclic-static, and cyclic with a period of unloading were exerted on the elastic membrane. Duration of cyclic loading and static loading was 5 and 12 hours respectively.
Results: The results illustrate that 10% cyclic stretch causes cell alignment but there were no significant proliferation differences between control and test group. Combined cyclic-static stretch reduced proliferation significantly while cyclic stretch with an unloading period increased cell proliferation significantly. At last, static stretch did not affect cell proliferation significantly.
Conclusion: Cell stretching regimes and post-loading duration are effective factors on cell proliferation.
Keywords
Tissue engineering
Static Stretch
Cyclic Stretch
Cell Proliferation
Human Mesenchymal Stem Cell