Study of the Expressions of IL-20R1 and IL-20R2 in C57BL/6 Mice Astroglial Cells

Authors
B. Abd Nikfarjam 1
M. Ebtekar 2
F. Sabouni 3
Z. Pourpak 4
M. Kheirandish 5
1 Ph.D. Candidate, Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Associate Professor, Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3 Assistant Professor, Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4 Professor, Immunology, Asthma and Allergy Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Assistant Professor, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Objective: Astrocytes are the most abundant glial cell type. They may promote or inhibit CNS inflammation depending on which cytokines are secreted. Astrocytes also have immune roles. IL-19, IL-20, and IL-24 activate a heterodimer receptor composed of the IL-20R1 α-chain and the IL-20R2 β-chain. It has long been considered that signaling by these receptor complexes affects immunological reactions, however the biological functions of IL-20R1 and IL-20R2 in the brain remain unclear. As the first step to address the role of these cytokine receptors in the brain, in this study we have researched the expressions of IL-20R1 and IL-20R2 in C57BL/6 mice astrocytes.
Methods: We examined expressions of IL-20R1 and IL-20R2 proteins in mice astroglial cells and in the 1321N1 astrocytoma cell line in response to MOG, LPS and GM-CSF by flow cytometry. The effect of LPS on mRNA expression of IL-20R1 and IL-20R2 was investigated by RT-PCR.
Results: We provide, for the first time, evidence that astrocytes expressed IL-20R1 and IL-20R2 mRNA not only in response to LPS stimulation but also in unstimulated astrocytes. We did not observe the expressions of IL-20R1 and IL-20R2 proteins in mice astroglial cells and the 1321N1 astrocytoma cell line.
Conclusions: IL-20R1 and IL-20R2 mRNA are constitutively expressed in astrocytes. Because the majority of neuropathological processes involve astrocytes and inflammatory cytokines, the results of this study, which are reported for the first time, have important implications for future research.

Keywords

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Pathobiology Reserach
Volume 15, Issue 4
February 2013
Pages 35-47