Nitric Oxide and Hydrogen Peroxide Production in Neutrophils and Monocytes of the Patients with Urinary Tract Infection Caused by E.coli before and after Ciprofloxacin Therapy

Antibiotics are widely used as bacteriostatic and bactericidal drugs in bacterial infections. Besides the respective interactions between the antibiotics and bacteria and between the immune system and bacteria, antibiotics also interact directly with the immune system and have various immunomodulatory effects on phagocytosis, chemotaxis, cytokine production and endotoxine release.
In this study, monocytes and neutrophils were separated from the blood samples of the patients with urinary tract infection before and after the therapy with 500 mg ciprofloxacin tablets. The cells were cultured in the presence of ciprofloxacin and activators including IFN-γ and LPS or PMA (for in vitro evaluation) or only the activators (for ex vivo evaluation) . Supernatants of the cells were collected and then production of NO and H2O2 was measured.
The effect of ciprofloxacin on NO and H2O2 production by monocytes and neutrophils in the patients with urinary tract infection caused by E. coli (E.Coli) was evaluated.The results were compared to an age- and sex-matched normal population. Peripheral blood samples from 45 patients with uUTI were collected at the time of diagnosis and at the end of the treatment.
The results showed that NO and H2O2 production was significantly increased in the patients comparison to the control group, before and after the treatment (P<0.05). NO level in the post treatment group was also significantly raised compared to the pre-treatment group (P<0.05), but H2O2 level was not significantly altered (P>0.05).
The results showed that the results of in vitro study was different from ex vivo. and alteration was not different between pre-treatment and post treatment groups significantly (P>0.05) .