Effect of hydroalcoholic extract of Lavender on apoptosis of liver cancer cells (HepG2)

Document Type : Original Research

Authors
M. Rezaei 1
E. Rajabbeigi 2
1 Department of Biochemistry-Biophysics, Faculty of Advance Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 2. Department of Developmental Biology, Faculty of Advance Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract
Objective: Despite many advances in cancer treatment and control, significant deficiencies remain and the rate of cancer growth is increasing. Due to the side effects of using chemicals and radiation, the use of herbs can have fewer side effects for the patient. The aim of this study was to investigate the potential anticancer effects of alcoholic extract of Lavender on inhibition and growth of HepG2 cell lines.

Materials and Methods: In this study, the effect of alcoholic extract of lavender (10,100,1000 and 1 μg / ml) on HepG2 liver cancer cell line was studied. MTT assay was used to evaluate the toxicity of lavender extract and cell viability. Cell cycle changes were assessed using a flow cytometer.

Oxygen-free species production and membrane lipid peroxidation were also measured.

Results: Results showed that alcoholic extract of Lavender has anti-cancer effect on HepG2 cell line. After confirming the effect of lavender extract on cell cycle, the results obtained from the evaluation of membrane lipid peroxidation and production of free reactive species were also significant.
Conclusion: According to the results of this study, the alcoholic extract of Lavender was reported as a potential anticancer agent and is one of the therapeutic strategies in liver cancer.

Keywords

Subjects

1.Dolatkhah R, Somi MH, Jabbarpour Bonyadi M, Kermani I, Farassati F, Dastgiri S. Colorectal cancer in Iran :molecular epidemiology and screening strategies. Cancer Epidemiol. 2015; 64(30):1-10
2. Kooti W, Servatyari K, Behzadifar M, Asadi M, Sadeghi F, Nouri B, Zare H. effective medicinal plantin cancer treatment,part two :Review stady. Evid-Based Integr Med. 2017;22(4):982_995
3.Ferlay J, SHin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of world wide burned of cancer in 2008:GLOBOCAN 2008. Int J Cancer. 2010;127(12):2893_2917
4.Hamid A, Goltom I, Rim T, ZHang Y, ZHang ZH. Aflatoxin B1 induced hepatocellular carcinoma in developing countries :Geographical distribution mechanism of action and prevention. Oncol Lett. 2013;5(4):1087_1092
5.Nordenstedt H, White DL, EI_serag HB. the changing pattern of epidemiology in hepatocellular carcinoma .Dig Liver Dis. 2010;42(3):206_214
6. Desai AG, Qazi GN, Ganju RK, EL_Tamer M, Singh J, Saxena AK, Bedi YS, Taneja SC, Bhat HK. Medicinal plants and cancer chemoprevention . Curr Drug Metab. 2008; 9(7):581_591
7 .Majd A, Mehrabian S, Jonoobi P, Modaresi A. A comparative study of anti_mutation and anti_carcinogenic properties of Rosemary and Lavender during their different developmental stages. J Med Microbiol. 2011;5(3):61_67
8. Dalilian S, Heidari kashl S, Zamanian Azodi M, Omidi R, Roeintan R, Hoseini R. The Evaluation of Lavender Aqueous Extract on Human Fibroblast Cells. Sjimu. 2013; 21(1):143-149
9. Julia R, Barret T. Liver cancer and Aflatoxin :new information from the Kenyan outbreak. Environ Health Perspect. 2005;113(12):837_838
10.Cardia GFE, Silva_Filho SE, Silva EL, Uchida NS, Cavalcante HAO, Cassarotti LL, Salvadego VEC, Spironello RA, Bersani_Amado CA, Cuman RKN. Effect of Lavander (Lavandula angustifolia) Essential oil on Acute infhammatory Rsponse. Evid Based complement Alternat Med. 2018;1413940
11. Amiri A, Tayarani-Najaran Z, Karimi G, Mousavi S. Evaluation of Lavandula angustifolia cytotoxic and apoptotic effects on human cervical cancer cell line (Hela) in compare with normal cells. Res Pharm Sci. 2012;7(5): 141.
12. Zhao Y, Chen R, Wang Y, Qing C, Wang W, Yang Y. In Vitro and In Vivo Efficacy Studies of Lavender angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer. Integr Cancer Ther. 2017; 16(2): 215–226
13. Dalilan, S., Rezaei-Tavaniri, M., Nabiuni, M., Heidari-Keshel, S., Azodi, M., Zali, H. Aqueous Extract of Lavender angustifolia Inhibits Lymphocytes Proliferation of Hodgkin's Lymphoma Patients. Iran J Cancer Prev. 2013; 6(4): 201–208.
14. Hamzeh, S., Safari-Faramani, R., Khatony A. Effects of Aromatherapy with Lavender and Peppermint Essential Oils on the Sleep Quality of Cancer Patients: A Randomized Controlled Trial. Evid Based Complement Alternat Med. 2020; 2020: 7480204. doi: 10.1155/2020/7480204
15. Ferhi S, Santaniello S, Zerizer S, Cruciani S, Fadda A, Sanna D, Dore A, Maioli M, Hallewin G. Total phenols from Grape leaves counteract cell proliferation and Modulate Apoptosis Related Gene Expression in MCF_7 and HepG2 Human cancer line. Molecules. 2019;24(3) :612
16. Sandhya V, Nair G, Hettihewa M, Rupasinghe V. Apoptotic and Inhibitory Effects on cell proliferation of Hepatocellular carcinoma HepG2 cell by Methanol leaf Extract of costus speciosus. Biomed Res Int. 2014;10(1)
17. Slimi S, Jallouli M, Gharbi N, Marzouki L. Hepatoprotective and Renoprotective effects of lavender Essential oils Against Malathion_Induced oxidative stress in young male Mice. J Med Food. 2015;18(10) :1103_1111
18. Shadkhast M, Bigham-Sadegh A, Nourani H, Eslami F. Histopathological and macroscopical evaluation of Lavandulla officinalis effects on wound healing in rabbit model. Iran J Vet Surg. 2014;8( 2): 91_96
19. Mehdinezhad Doghikolayi S, Mohammadi M. The antioxidant and cytotoxic effects of Lavandulla anguostifoulya on the 8305C cell line. New Cell Mol Biotech J. 2018; 8(32): 91-98 (in persian)
20. Zamanian-Azodi M, Rezaie-Tavirani M, Heidari-Kashal S, Kalantari Sh, Dailian S, Zali H. Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract. Gastroenterol Hepatol Bed Bench; 2012; 5(1): 35-42
21. Zhang Z, Chen H, Chan KK, Budd T, Ganapathi R. Gas chromatographic-mass spectrometric analysis of perillyl alcohol and metabolites in plasma. Journal of Chromatography B: Biomedical Sciences and Applications. 1999;728(1):85–95
22. Prashar A., Lock I, Evans CS. Cytotoxicity of lavender oil and its major components to human skin cells. Cell Proliferat; 2004; 37 (3): 221-229
23. Zhao Y, Chen R, Wang Y, Qing Ch, Wang W, Yang Y. In Vitro and In Vivo Efficacy Studies of Lavender angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer. Integr cancer Ther. 2017; 16(2):215-226
24. Kim H, Tu H, Ren D, Takeuchi O, Jeffers J, Zambetti G, Hsieh J, Cheng E. Stepwise activation of BAX and BAK by tBID, BIM, and PUMA initiates mitochondrial apoptosis. Mol Cell. 2009;36:487–499.
25. Lin Y, Cui H, Xu H, Yue L, Xu HJiang L. Jolkinolide B induces apoptosis in MDA_MB231 cells through inhibition of the PI3K/AKt signaling pathway. oncol Rep .2012;27(6):1996
26. Koulivand H, Khaleghi Ghadiri M, Gorji A. Lavender and the Nervous System. Evid Based Complement Alternat Med. 2013;2013; 681304.
27. Sebai H, Slimi S, Rtibi K, Souli A, GHarbi N, Sakly M. Lavander (lavandula stoechas L.)essential oils attenuate hyperglycemia and protect against oxidative stress in alloxan_induced diabetic rats. Lipids Health Dis. 2013;189(12)
28. Chen A, Huang X, Xue Z, Cao D, Huang K, Chen J, Pan Y, Gao Y. the Role of p21 in Apoptosis proliferation cell cycle Arrest and Antioxidant Activity in UVB irradiated Human H cate keratinocytes. Med Sci Monit Basic Res. 2015;30(21):86_95
29. Farshori N, Al_Sheddi ES, Al_Oqail MM, Hassan WH, AL_Khedhairy AA, Musarrat J, Siddiqui MA. Hepatoprotective potential of lavandula cornopifolia extracts against ethanol induced oxidative stress mediated cytotoxicity in HepG2 cell.Toxicol Ind Healt. 2015;31(8) :727_37