Evaluation the level of IL-1β and TNF-α and joint friction in the non-traumatic model of knee osteoarthrosis in Guinea Pig

Document Type : Original Research

Authors
M. Ahmadi-fakhre
G. Torkaman
R. ravanbod
Physical Therapy Department, Faculty of Medical Sciences, Tarbiat Modares University
Abstract
Introduction: Osteoarthritis is the most common type of arthritis and is one of the most common causes of pain and disability in adults. In the present study we investigated the level of inflammatory cytokines IL-1β and TNF-α and the amount of joint friction after non-traumatic model of knee osteoarthrosis in guinea pig.

Materials & Methods: Twenty-four matured albino male Dunkin-Hartley guinea pigs were assigned randomly into two groups; osteoarthrosis (OA) and control (n=12 in each group). The non-traumatic OA was induced by intra-articular injection of mono iodoacetate (MIA, 3 mg/kg) in the knee joint. Joint circumstance and weight of subjects were measured initially and after thirty days of MIA injection. After scarifying the animals on day 30, the level of IL-1β and TNF-α in synovial membrane and knee joint friction were measured. Control group received no intervention and all evaluations were performed on the normal knee joint.

Results: The induction of OA caused a significant increase in the joint circumstance compared to control group (P<0.05). 30 days after induced OA, the knee joint friction in flexion and extension movement was significantly increased compared to control group. In OA group, the level of IL-1β and TNF-α showed a significant increase compared to control group (P<0.05). There was a positive correlation between IL-1β, TNF-α and knee joint friction (P<0.01).

Discussion: Non-traumatic osteoarthritis may increase the expression of inflammatory cytokines and joint friction, which in this condition the joint movement can aggravate cartilage damage.

Keywords

Subjects

References
۱. Davatchi F, Jamshidi A-R, Banihashemi AT, Gholami J, Forouzanfar MH, Akhlaghi M, et al. WHO-ILAR COPCORD study (stage 1, urban study) in Iran. The Journal of rheumatology. 2008;35: 1384-1390. ]Persian[
۲. Control CfD, Prevention. Racial/ethnic differences in the prevalence and impact of doctor-diagnosed arthritis--United States, 2002. MMWR Morbidity and mortality weekly report. 2005;54(5):119.
۳. Goldring MB, Otero M. Inflammation in osteoarthritis. Current opinioin in rheumatology. 2011;23(5):471-478.
۴. Kapoor M, Martel-Pelletier J, Lajeunesse D, Pelletier J-P, Fahmi H. Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nature Reviews Rheumatology. 2011;7(1):33.
۵. Liu-Bryan R. Synovium and the innate inflammatory network in osteoarthritis progression. Current rheumatology reports. 2013;15(5):323.
.۶ Elsaid K, Jay G, Warman M, Rhee D, Chichester C. Association of articular cartilage degradation and loss of boundary‐lubricating ability of synovial fluid following injury and inflammatory arthritis. Arthritis & Rheumatism. 2005;52(6):1746-55.
۷. DuRaine G, Neu CP, Chan SM, Komvopoulos K, June RK, Reddi AH. Regulation of the friction coefficient of articular cartilage by TGF‐β1 and IL‐1β. Journal of Orthopaedic Research. 2009;27(2):249-56.
۸. Andrades JA, Motaung SC, Jiménez-Palomo P, Claros S, López-Puerta JM, Becerra J, et al. Induction of superficial zone protein (SZP)/lubricin/PRG 4 in muscle-derived mesenchymal stem/progenitor cells by transforming growth factor-β1 and bone morphogenetic protein-7. Arthritis research & therapy. 2012;14(2):R72.
۹. Forsey RW, Fisher J, Thompson J, Stone MH, Bell C, Ingham E. The effect of hyaluronic acid and phospholipid based lubricants on friction within a human cartilage damage model. Biomaterials. 2006;27(26):4581-90.
۱۰. Nordin M, Frankel VH. Basic biomechanics of the musculoskeletal system: Lippincott Williams & Wilkins; 2001.
۱۱. Cooke A, Dowson D, Wright V. The rheology of synovial fluid and some potential synthetic lubricants for degenerate synovial joints. Engineering in Medicine. 1978;7(2):66-72.
۱۲. Jay GD, Torres JR, Rhee DK, Helminen HJ, Hytinnen MM, Cha CJ, et al. Association between friction and wear in diarthrodial joints lacking lubricin. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology. 2007;56(11):3662-9.
۱۳. Teeple E, Jay GD, Elsaid KA, Fleming BC. Animal models of osteoarthritis: challenges of model selection and analysis. The AAPS journal. 2013;15(2):438-46.
14. Teeple E, Fleming BC, Mechrefe AP, Crisco JJ, Brady MF, Jay GD. Frictional properties of Hartley guinea pig knees with and without proteolytic disruption of the articular surfaces. Osteoarthritis Cartilage 2007;15:309-315.
15. Combe R, Bramwell S, Field MJ. The monosodium iodoacetate model of osteoarthritis: a model of chronic nociceptive pain in rats? Neuroscience letters. 2004;370(2-3):236-40.
16. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicologic pathology. 2003;31(6):619-24.
17. Ravanbod R, Torkaman G, Esteki A. Biotribological and biomechanical changes after experimental haemarthrosis in the rabbit knee. Haemophilia 2011;17:124-133.
18. Ravanbod R, Torkaman G, Esteki A. Comparison between pulsed ultrasound and low level laser therapy on experimental haemarthrosis. Haemophilia. 2013;19(3):420-5.
19. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicologic pathology. 2003;31(6):619-24.
20. Chang K-V, Hung C-Y, Aliwarga F, Wang T-G, Han D-S, Chen W-S. Comparative effectiveness of platelet-rich plasma injections for treating knee joint cartilage degenerative pathology: a systematic review and meta-analysis. Archives of physical medicine and rehabilitation. 2014;95(3):562-75.
21. Goldring SR, Goldring MB. The role of cytokines in cartilage matrix degeneration in osteoarthritis. Clinical Orthopaedics and Related Research (1976-2007). 2004;427:S27-S36.

22. Sokolove J, Lepus CM. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Therapeutic advances in musculoskeletal disease. 2013;5(2):77-94.
23 Wang M, Sampson ER, Jin H, Li J, Ke QH , Im HJ, Chen D. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Research and Therapy. 2013;15:R5.
24. Jones A, Keohan C, Sheldon R. Regulation of lubricin expression and metabolism in synovial joints. Eur Cell Mater. 2006;12(suppl 1):17.
Pathobiology Reserach
Volume 23, Issue 5
April 2021
Pages 7-15