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Evaluation of Cytotoxicity Activity of Salmonella Typhimurium Protein Fractions on Melanoma BL16 Cancer Cell Line
Minoo Najafi1, Neda Soleimani1*, Atoosa Aliahmadi2
1- Departments of Microbiology and Microbial Biotechnology and Nanobiotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2- Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Background and target: Salmonella typhimurium is a gram-negative rod-forming bacterium belonging to the family Enterobacteriaceae and Is one of the most common foodborne pathogens, that With several pathogenic factors, including toxins, Secretory proteins, and secretory system T3SS(That Causes fluid secretion And inflammation), Etc It can a suitable candidate for this stud. The purpose of this study, Evaluation of the cytotoxicity effect of bacterial protein fractions on growth and proliferation of melanoma cancer cells, which is the most resistant type of skin cancer.
materials and methods: In this experimental study, the cancer cell line (BL16) melanoma was used. Different bacterial fractions were prepared by the ammonium sulfate method. Interaction of cancer cells with different concentrations of Salmonella typhimurium fractions was studied. Cell proliferation was assessed by MTT assay at 24 and 48 hours.
findings: MTT test results with fractions (Bacteria lysate in culture medium, 80% deposition of lysate proteins, 30% deposition of lysate proteins, 80% deposition on culture media, and 30% deposition on culture media) The highest effect was observed at concentrations of 9.25,30, 8, 72, 10.75 μg / ml, respectively.
Discussion and conclusion: Results show that bacterial fractions of Salmonella typhimurium have high toxicity and lethal effect on melanoma cancer cells. These compounds can be suggested and used as an alternative or complementary to cancer therapy.
key words: Cytoplasmic extract, Media, Salmonella typhimurium, melanoma
Pathobiology Reserach