Isolation of a bacteriophage with lytic activity on enteropathogenic Escherichia coli from a waste water source in Tehran

Document Type : Original Research

Authors
K. Esmaeili Fard Barzegar 1
S. Najar-peerayeh 1
R. Mazaheri Nezhad Fard 2
B. Bakhshi 3
1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Associated professorDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IraFood Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Professor, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Introduction: Enteropathogenic Escherichia coli (EPEC) strains cause a gastrointestinal disease in the developing countries. Over the years, antimicrobial resistance of EPEC have been a major issue. Bacteriophages have been a therapeutic option for bacterial infection. Our aim was to assay lytic activity of a siphophage on an EPEC strain. Materials and Method: EPEC strain ATCC 43887 was used for phage isolation using by double layer agar method. Bacteriophage morphology was visualized by transmission electron microscope. Result: a siphophage was detected by transmission electron microscope images. The phage formed small clear circular plaques. The results of lytic activity of siphophage on reference EPEC strain showed the phage could lyse the strain. Conclusion: the phage had lytic activity on reference EPEC strain. The phage belonged to siphoviridae family. It look the phage can lyse clinical EPEC strains.

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