Synergistic Antibacterial Activity of synthesized Graphene Oxide/Chitosan nanocomposite with Rosmarinus officinalis L. Essential Oil against Multidrug-Resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli isolates

Document Type : Original Research

Authors
S. Honarmand Jahromi 1
M. Mahdavi-Ourtakand 2
A. Nasrollahi Omran 3
Z. Gerami Moazam 4
1 Department of Microbiology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
2 Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
3 Department of Medical Mycology, Faculty of Medicine, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
4 Department of biology, College of basic science and technology innovation, E-Campus Branch, Islamic Azad University, Tehran, Iran
Abstract
Treatment of infections caused by multidrug-resistant bacteria has become a global challenge. The combined therapies involve the simultaneous use of two or more biological agents with different mechanisms of action, which are more effective than traditional treatments for diseases that act only in one way. The aim of this study was synergistic antibacterial activity of synthesized graphene oxide/chitosan (GO/CS) nanocomposite with Rosmarinus officinalis L. essential oil against multidrug-resistant (MDR) bacteria. R. officinalis essential oil was extracted and its chemical compounds were analyzed by gas chromatography and mass spectrometry. The GO/CS nanocomposite was synthesized. The size and structure of the synthesized nanoparticles were evaluated by EDS, XRD, FE-SEM, and FTIR analysis. Antibacterial activity of chitosan, graphene oxide, GO/CS nanocomposite and R. officinalis essential oil was studied by broth microdilution method against 5 MDR isolates of A. baumannii, P. aeruginosa and E. coli. The antimicrobial interaction of the essential oil and GO/CS composite was studied by checkerboard titration method. The results showed that chitosan, graphene oxide and GO/CS had no antimicrobial activity in the studied concentrations. The MIC of R. officinalis essential oil was obtained between 0.12-256 μl/ml. R. officinalis essential oil in combination with GO/CS nanocomposite had a synergistic effect against 5 isolates of P. aeruginosa and 2 isolates of A. baumannii, and caused an additive effect against two isolates of E. coli. Based on the findings of this study, this combination can be effective against some MDR isolates and could be used to treat infections caused by these isolates.

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1. Mostofi S, Mirnejad R, and Masjedian F, Multi-drug resistance in Acinetobacter baumannii strains isolated from clinical specimens from three hospitals in Tehran-Iran. African Journal of Microbiology Research. 2011; 5(21): 3579-82.
2. Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D, Rice LB, Scheld M, Spellberg B, Bartlett J. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical infectious diseases. 2009;48(1):1-2.
3. Pieklarz K, Tylman M, Modrzejewska Z. Applications of chitosan–graphene oxide nanocomposites in medical science: a review. Progress on Chemistry and Application of Chitin and its Derivatives. 2018;23:5-24.
4. Peers S, Montembault A, Ladavière C. Chitosan hydrogels for sustained drug delivery. Journal of Controlled Release. 2020;326:150-63.
5. Ahmed J, Mulla M, Maniruzzaman M. Rheological and dielectric behavior of 3D-printable chitosan/graphene oxide hydrogels. ACS Biomaterials Science & Engineering. 2019;6(1):88-99.
6. Dinh DA, Hui KS, Hui KN, Cho YR, Zhou W, Hong X, Chun HH. Green synthesis of high conductivity silver nanoparticle-reduced graphene oxide composite films. Applied surface science. 2014;298:62-7.
7. Akhavan O, Ghaderi E. Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS nano. 2010;4(10):5731-6
8. Gu Z, Zhu S, Yan L, Zhao F, Zhao Y. Graphene‐based smart platforms for combined Cancer therapy. Advanced Materials. 2019;31(9):1800662.
9. Maiti D, Tong X, Mou X, Yang K. Carbon-based nanomaterials for biomedical applications: a recent study. Frontiers in pharmacology. 2019;9:1401.
10. Gurunathan S, Kim JH. Synthesis, toxicity, biocompatibility, and biomedical applications of graphene and graphene-related materials. International journal of nanomedicine. 2016;11:1927.
11. Valencia AM, Valencia CH, Zuluaga F, Grande-Tovar CD. Dataset on in-vitro study of chitosan-graphene oxide films for regenerative medicine. Data in Brief. 2021;39:107472.
12. Zhang L, Li X, Shi C, Ran G, Peng Y, Zeng S, He Y. Biocompatibility and angiogenic effect of chitosan/graphene oxide hydrogel scaffolds on EPCs. Stem Cells International. 2021;2021.
13. Khalil WF, El-Sayyad GS, El Rouby WM, Sadek MA, Farghali AA, El-Batal AI. Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria. International Journal of Biological Macromolecules. 2020;164:1370-83.
14. Martins AF, Facchi SP, Follmann HD, Pereira AG, Rubira AF, Muniz EC. Antimicrobial activity of chitosan derivatives containing N-quaternized moieties in its backbone: a review. International Journal of Molecular Sciences. 2014;15(11):20800-32.
15. Borges RS, Ortiz BL, Pereira AC, Keita H, Carvalho JC. Rosmarinus officinalis essential oil: A review of its phytochemistry, anti-inflammatory activity, and mechanisms of action involved. Journal of ethnopharmacology. 2019;229:29-45.
16. El Kharraf S, El-Guendouz S, Farah A, Bennani B, Mateus MC, Miguel MG. Hydrodistillation and simultaneous hydrodistillation-steam distillation of Rosmarinus officinalis and Origanum compactum: Antioxidant, anti-inflammatory, and antibacterial effect of the essential oils. Industrial Crops and Products. 2021;168:113591.
17. Jafari-sales A, Pashazadeh M. Study of chemical composition and antimicrobial properties of Rosemary (Rosmarinus officinalis) essential oil on Staphylococcus aureus and Escherichia coli in vitro. International Journal of Life Sciences and Biotechnology. 2020;3(1):62-9.
18. CLSI Performance standards for antimicrobial susceptibility testing; 31ed. CLSI document M100. Clinical and Laboratory Standards Institute, Wayne, PA. 2021.
19. Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. Carol Stream: Allured publishing corporation; 2007.
20. Hummers W, & Offeman R, Graphite oxide (GO) was prepared using the well-known Hummers method described by Hummers. J Am Chem Soc. 1958;80: 1339-1345.
21. Sabzevari M, Cree DE, Wilson LD. Graphene oxide–chitosan composite material for treatment of a model dye effluent. ACS omega. 2018;3(10):13045-54.
22. Odds FC. Synergy, antagonism, and what the chequerboard puts between them. Journal of Antimicrobial Chemotherapy. 2003;52(1):1-.
23. Biavatti MW. Synergy: an old wisdom, a new paradigm for pharmacotherapy. Brazilian Journal of Pharmaceutical Sciences. 2009;45:371-8.
24. Grande CD, Mangadlao J, Fan J, De Leon A, Delgado‐Ospina J, Rojas JG, Rodrigues DF, Advincula R. Chitosan Cross‐Linked Graphene Oxide Nanocomposite Films with Antimicrobial Activity for Application in Food Industry. InMacromolecular symposia 2017;374(1):1600114).
25. Chowdhuri AR, Tripathy S, Chandra S, Roy S, Sahu SK. A ZnO decorated chitosan–graphene oxide nanocomposite shows significantly enhanced antimicrobial activity with ROS generation. Rsc Advances. 2015;5(61):49420-8.
26. Jiang Y, Gong JL, Zeng GM, Ou XM, Chang YN, Deng CH, Zhang J, Liu HY, Huang SY. Magnetic chitosan–graphene oxide composite for anti-microbial and dye removal applications. International journal of biological macromolecules. 2016;82:702-10.
27. Chen Y, Du M, Yu J, Rao L, Chen X, Chen Z. Nanobiohybrids: a synergistic integration of bacteria and nanomaterials in cancer therapy. BIO Integration. 2020;1(1):25-36.
28. Feng W, Wang Z. Biomedical applications of chitosan-graphene oxide nanocomposites. Iscience. 2022;25(1).
29. Jubair N, Rajagopal M, Chinnappan S, Abdullah NB, Fatima A. Review on the antibacterial mechanism of plant-derived compounds against multidrug-resistant bacteria (MDR). Evidence-Based Complementary and Alternative Medicine. 2021;2021.
30. Onyancha W, Ali MI, Sharma G, Moin S. Synergistic potential of herbal plants and conventional antibiotics against multidrug-resistant bacteria. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2021;13(1):13-21.
31. Chitin and its Derivatives. 2018;23:5-24.
32. Sundar K, Harikarthick V, Karthika VS, Ravindran A. Preparation of chitosan-graphene oxide nanocomposite and evaluation of its antimicrobial activity. Journal of Bionanoscience. 2014;8(3):207-12.
33. Li W, Jiang T, Pu Y, Jiao X, Tan W, Qin S. Glucose biosensor using fluorescence quenching with chitosan‐modified graphene oxide. Micro & Nano Letters. 2019;14(3):344-8.