The effect of hyperglycemia on catalase activity and glutathione levels of liver in streptozotocin-induced diabetes mellitus in rat

Document Type : Original Research

Author
M. T. Mohammadi
Baqiyatallah University of Medical Sciences
Abstract
Introduction: The antioxidant enzymes and antioxidant capacity are affected by hyperglycemia in the different tissues of human body during diabetes mellitus (DM). Hence, in the present study we measured the activity of catalase and glutathione content in the livers of streptozotocin-induced diabetic rats.

Methods: Male Wistar rats were randomly divided into normal and diabetic groups (n=6). To induce DM, a single intravenous injection of streptozotocin was used (45 mg/kg). Blood glucose of rats was measured at the beginning and termination of study. Likewise, the activity of catalase and the content of glutathione were determined in the livers at termination of the study.

Results: Induction of DM increased blood glucose of the diabetic rats to 559 ± 35 mg/dL. This value did not change during the test (610 ± 17 mg/dL) in diabetic rats. Diabetes also increased the catalase activity in the livers of diabetic rats compared to normal group. Likewise, glutathione content increased in the livers of diabetic animals compared to normal rats.

Conclusion: Our findings revealed that the activity of antioxidant enzymes as well as antioxidant capacity of liver may be increased as a compensatory response to confront the tissue oxidative stress for the determined time during diabetes. It is suggested, if the period of diabetes be prolonged, this compensatory response may be weakened.

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Pathobiology Reserach
Volume 26, Issue 3
September 2023
Pages 37-41