Development of a Heterotopic Zebrafish Model for Real-Time Observation of Retinoblastoma Tumor Growth

Document Type : Original Research

Authors
L. Satraian 1
S. Fallah 2
1 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Abstract
Introduction: Retinoblastoma, the most prevalent pediatric eye cancer, arises from mutations in the RB1 gene, leading to the uncontrolled proliferation of retinal cells. This study introduces a heterotopic retinoblastoma model utilizing zebrafish, focusing on injecting the Y79 retinoblastoma cell line into the vitreous cavity for real-time tumor observation. Methods: By leveraging the transparent embryos and rapid eye development of zebrafish, we tracked the establishment and growth of fluorescently labeled tumors. Results: Results confirm tumor formation within three days, underscoring the model's relevance for in vivo studies. The zebrafish model capitalizes on the ease of maintenance, transparency for direct visualization, and genetic tractability, offering significant potential for high-throughput screening and therapeutic assessments. Conclusion: As the field progresses, this model promises to enhance our understanding of retinoblastoma biology and facilitate the discovery of effective treatments, addressing the critical need for innovative approaches in pediatric oncology

Keywords

Subjects

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Pathobiology Reserach
Volume 27, Issue 1
May 2024
Pages 45-52