The effect of aerobic training on some indicators of vascular endothelial function in none-active adult obese women

Document Type : Original Research

Authors
1 Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran
2 Department of Exercise physiology, Saveh Branch, Islamic Azad University, Saveh, Iran
3 Department of Exercise Physiology, Semnan Branch, Islamic Azad University, Semnan, Iran
Abstract
Introduction: Clinical evidence has supported vascular endothelial dysfunction in the presence of obesity. The aim of this study was to assess the effect of aerobic training on nitric oxide (NO) level, total antioxidant capacity (TAC) and homocysteine as markers of vascular endothelial function in inactive adult obese females.

Methods: For this purpose, 26 sedentary adult obese females were randomly divided into of aerobic (8 weeks, 3days/weekly, n= 12) and control (no training, n = 12) groups. Fasting serum levels of NO, TAC and homocysteine were measured before training program and 48 hours after lasting exercise session in both groups. Independent t-test was used to compare the pre-tests (baseline) between two groups and paired t-test was used to determine intra-group changes.

Results: No significant difference were observed in each variable between groups at baseline (p>0.05). Aerobic training resulted in significant decrease in homocysteine and increase in NO and TAC in aerobic group (p<0.05). All variables remained without change in control group (p>0.05).

Conclusion: Based on these results, it is concluded that regular aerobic exercise improves vascular endothelial function in obese adult women.

Keywords

Subjects


1. Li J, Ge Z, Li C, Ran H, Zhang Y, Xiang Y. METRNL exerts cytoprotective effects on EPCs via regulation of the E2F1-TXNIP axis in obese limb ischemia. Cell Signal. 2025 Feb; 126:111528.
2. Kobayasi R, Akamine EH, Davel AP, Rodrigues MA, Carvalho CR, Rossoni LV. Oxidative stress and inflammatory mediators contribute to endothelial dysfunction in high-fat diet-induced obesity in mice. J Hypertens. 2010 Oct; 28(10):2111-9.
3. Li YS, Ren HC, Li H, Xing M, Cao JH. From oxidative stress to metabolic dysfunction: The role of TRPM2. Int J Biol Macromol. 2025 Jan; 284(Pt 1):138081.
4. Batinac T, Batičić L, Kršek A, Knežević D, Marcucci E, Sotošek V, Ćurko-Cofek B. Endothelial Dysfunction and Cardiovascular Disease: Hyperbaric Oxygen Therapy as an Emerging Therapeutic Modality? J Cardiovasc Dev Dis. 2024 Dec 19; 11(12):408.
5. Mohammadi S, Lotfi K, Mirzaei S, Asadi A, Akhlaghi M, Saneei P. Dietary total antioxidant capacity in relation to metabolic health status in overweight and obese adolescents. Nutr J. 2022 Aug 30; 21(1):54.
6. Powers SK, Jackson MJ.Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev. 2008 Oct; 88(4): 1243-76.
7. Unt E, Zilmer K, Magi A, Kullisaar T, Kairane C, Zilmer M. Homocysteine status in former toplevel male athletes: possible effect of physicalactivity and physical fitness. Scan J Med Sci Sports 2007; 18(3): 360-6.
8. Dankner R, Chetrit A, Dror GK, Sela BA. Physical activity is inversely associated with total homocysteine levels, independent of C677T MTHFR genotype and plasma B vitamins. Age (Dordr) 2007; 29(4): 219-27.
9. Gallistl S, Sudi KM, Erwa W, Aigner R, Borkenstein M. Determinants of homocysteine during weight reduction in obese children and adolescents. Metabolism 2001; 50(10): 1220-3.
10. Green DJ, Maiorana AO, Driscoll G, Taylor R. Effect of exercise training on endothelium-derived nitric oxide function in humans. J Physiol. 2004; 561(1):1-25.
11. Gantner BN, LaFond KM, Bonini MG. Nitric oxide in cellular adaptation and disease. Redox Biol. 2020 Jul; 34:101550.
12. Ficicilar H1, Zergeroglu AM, Ersoz G, Erdogan A, Ozdemir S, Tekin D. The effects of short-term training on platelet functions and total antioxidant capacity in rats. Physiol Res. 2006; 55(2):151-6.
13. Azizbeigi K, Azarbayjani MA, Peeri M, Agha-alinejad H, Stannard S. The effect of progressive resistance training on oxidative stress and antioxidant enzyme activity in erythrocytes in untrained men. Int J Sport Nutr Exerc Metab. 2013 Jun; 23(3):230-8.
14. Housaini SL, Eizadi M. The effect of 8 weeks TRX training on glutathione peroxidase (GPx) and hydrogen peroxide (H2O2) in sedentary middle-aged obese men. Razi J Medl Sci. 2020; 27(5): 210-219.
15. Soori R, Choopani S, Falahian N, Ramezankhani A. Effect of Physical Activity on Serum Homocysteine Levels in Obese and Overweight Women. Quarterly of the Horizon of Medical Sciences. 2016; 22(4): 307-12).
16. Zhong X, He R, You S, Liu B, Wang X, Mao J. The Roles of Aerobic Exercise and Folate Supplementation in Hyperhomocysteinemia-Accelerated Atherosclerosis. Acta Cardiol Sin. 2023 Mar; 39(2):309-318.
17. Shing CLH, Bond B, Moreau KL, Coombes JS, Taylor JL. The therapeutic role of exercise training during menopause for reducing vascular disease. Exp Physiol. 2024 Nov 19. doi: 10.1113/EP092191. Epub ahead of print.
18. Ali Nouri H, Ghofrani M. The effect of eight weeks of practice High intensity interval (HIT) and Moderate-Intensity Continuous Training (MICT) on Adropin, Nitric Oxide (NO) and Blood pressure in obese boys. Journal of Sport Sciences &Educational Applied Researcher without Border. 2017; 3(10): 42-65.
19. Hejazi M, nezamdoost Z, Saghebjoo M. Effect of Twelve Weeks of Aerobic Training on Serum Levels of Leptin, Vaspin and Some Indicators of Oxidative Stress in Obese Middle-Aged Women. Iranian Journal of Endocrinology and Metabolism. 2014; 16 (2): 111-118.
20. Etemad Z, Nikbakht H, Azarbaijani M A, Gholami M. Concentrations of homocysteine and CRP after 8 weeks of resistance training circle with different rest intervals. SJKU 2017; 22 (1): 107-119.
21. Ghardashi Afousi A, Gaeini A, Gholami Borujeni B. The effect of aerobic interval training on endothelial vasculature function in type 2 diabetes patient. IJRN. 2016; 2 (3): 27-39.
22. Usefpor M, Ghasemnian A A, Rahmani A. The Effect of a period of high intensive interval training on total antioxidant capacity and level of liver tissue malondialdehyde in male Wistar rats. SJKU. 2017; 22 (5): 103-110.
23. Subaşı SS, Gelecek N, Aksakoğlu G, Omret M. Effects of two different exercise trainings on plasma homocysteine levels and other cardiovascular disease risks. Türk J Biochem. 2012; 37(3):303-14.
24. Harvey PJ, Picton PE, Su WS, Morris BL, Notarius CF, Floras JS. Exercise as an alternative to oral estrogen for amelioration of endothelial dysfunction in postmenopausal women. Am Heart J. 2005; 149(2): 291-7.
25. Green DJ, Maiorana AO, Driscoll G, Taylor R. Effect of exercise training on endothelium-derived nitric oxide function in humans. J Physiol. 2004; 561(1):1-25.
26. Higashi Y, Yoshizumi M. Exercise and endothelial function: role of endothelium-derived nitric oxide and oxidative stress in healthy subjects and hypertensive patients. Pharmacol Ther. 2004 Apr; 102(1):87-96.
27. Lloyd PG, Prior BM, Yang HT, Terjung RL. Angiogenic growth factor expression in rat skeletal muscle in response to exercise training. Am J Physiol Heart Circ Physiol. 2003 May; 284(5): 1668-78.
28. Xu Q. Role of heat shock proteins in atherosclerosis. Arterioscler Thromb Vasc Biol. 2002 Oct 1; 22(10):1547-59.
29. Perez-de-Arce K, Foncea R, Leighton F. Reactive oxygen species mediates homocysteine-induced mitochondrial biogenesis in human endothelial cells: modulation by antioxidants. Biochemical and Biophysical Research Communications. 2005; 338(2): 1103-9.
30. Alul RH, Wood M, Longo J, Marcotte AL, Campione AL, Moore MK, et al. Vitamin C protects low-density lipoprotein from homocysteine-mediated oxidation. Free Radical Biology and Medicine 2003; 34(7): 881-91.