The effect of resistance training on hepatic FTO gene expression, fasting glucose and insulin resistance in Wistar rats with type 2 diabetes

Document Type : Original Research

Author
H. Parsian
Department of Physical Education and Sport Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Abstract
Introduction: Clinical studies have supported the effective role of gluconeogenic in hepatic glucose release, especially in type 2 diabetics (T2D). This study was aimed to investigate the effect of resistance training on hepatocyte fat mass and obesity associated (FTO) gene expression as well as fasting glucose and insulin resistance in obese rats with type 2 diabetes.

Methods: For this purpose, T2D induced by 6 weeks high-fat diet (HFD) and intraperitoneal STZ injection in 14 male Wistar rats (220 ± 10 grams) and were then randomly divided into exercise (n = 7, resistance training) and control (n = 7) groups. The rat in exercise group were completed 8 weeks resistance training (5 times/weekly) and control rats remained non-training. 48 hours after exercise intervention, fasting blood glucose, serum insulin, insulin resistance and hepatocyte FTO expression were compared by independent t test between groups.

Results: Compared to control subjects, significant decrease were observed in fasting glucose (P = 0.001), insulin resistance (P = 0.001) and FTO expression (P = 0.024) and increase in serum insulin (P = 0.005) by exercise intervention in resistance group.

Conclusion:

Resistance training is accompanied by improving fasting glucose in T2D rats. This improvement may be attributed to the reduction of hepatocytes FTO expression and insulin resistance in response to resistance training.

Keywords

Subjects

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Pathobiology Reserach
Volume 28, Issue 1
February 2025
Pages 49-55