The Histological Effects of Diazinon on the Liver and Kidneys of Rats

Document Type : Original Research

Authors
M. Kalvandi
S. A. Hashemi
B. Tavakolifar
G. Bayat
M. Goudarzvand
Alborz University of Medical Sciences
Abstract
Introduction: Diazinon is an organophosphate pesticide that has widespread applications in both agriculture and household settings. Diazinon poisoning can have detrimental effects on the cardiovascular, gastrointestinal, and central nervous systems. The aim of this study was to investigate the effects of chronic exposure to diazinon on histological features of the liver and kidney of rats.

Materials and Methods: Twenty adult male Wistar rats, aged 10 - 12 weeks and weighing 150-200 grams, were purchased and divided into two groups of 10. In the treatment group, diazinon was administered at a dose of 20 mg/kg/day to each animal for 3 months, while the control group was maintained on a normal diet and drinking water. After 12 weeks of exposure, the animals were prepared for H & E staining, and the samples were examined under a light microscope.

Findings: The structural integrity of the liver and kidney was preserved in the diazinon-treated group compared to the control group, but mild effects of diazinon were observed in the liver tissue, such as foci of inflammation and hyperplasia of Kupffer cells, and in the kidney, such as the presence of epithelial cells in the distal tubule and cell detachment from the basement membrane.

Conclusion: Based on the results of this study, the rats exposed to diazinon did not show significant changes in histological features in the liver and kidney, and these changes were subtle, possibly indicating a need for higher doses or longer exposure durations to diazinon.

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1. Parween T, Jan S, Mahmooduzzafar S, Fatma T, Siddiqui ZH. Selective effect of pesticides on plant—A review. Critical reviews in food science and nutrition. 2016;56(1):160-79.
2. Büyükokuroğlu ME, Cemek M, Tosun M, Yürümez Y, Baş O, Yavuz Y. Dantrolene may prevent organophosphate-induced oxidative stress and muscle injury. Pesticide Biochemistry and Physiology. 2008;92(3):156-63.
3. Karami-Mohajeri S, Abdollahi M. Toxic influence of organophosphate, carbamate, and organochlorine pesticides on cellular metabolism of lipids, proteins, and carbohydrates: a systematic review. Human & experimental toxicology. 2011;30(9):1119-40.
4. Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food and chemical toxicology. 2010;48(12):3345-53.
5. Pratama D, Permata F, Widodo E, Fawzi H, editors. Molecular Study of Diazinon Toxicity Based on Inducible Nitric Oxide Synthase (iNOS) Expression in Hepatocytes and Kidney Histopathology of Rats (Rattus norvegicus). Journal of Physics: Conference Series; 2020: IOP Publishing.
6. Abdollahi M, Mostafalou S, Pournourmohammadi S, Shadnia S. Oxidative stress and cholinesterase inhibition in saliva and plasma of rats following subchronic exposure to malathion. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2004;137(1):29-34.
7. Ray DE, Richards P. The potential for toxic effects of chronic, low-dose exposure to organophosphates. Toxicology letters. 2001;120(1-3):343-51.
8. Chen Y. Organophosphate-induced brain damage: mechanisms, neuropsychiatric and neurological consequences, and potential therapeutic strategies. Neurotoxicology. 2012;33(3):391-400.
9. Lukaszewicz-Hussain A. Role of oxidative stress in organophosphate insecticide toxicity–Short review. Pesticide biochemistry and physiology. 2010;98(2):145-50.
10. Anbarkeh FR, Nikravesh MR, Jalali M, Sadeghnia HR, Sargazi Z, Mohammdzadeh L. Single dose effect of diazinon on biochemical parameters in testis tissue of adult rats and the protective effect of vitamin E. Iranian journal of reproductive medicine. 2014;12(11):731.
11. Khaksar MR, Rahimifard M, Baeeri M, Maqbool F, Navaei-Nigjeh M, Hassani S, et al. Protective effects of cerium oxide and yttrium oxide nanoparticles on reduction of oxidative stress induced by sub-acute exposure to diazinon in the rat pancreas. Journal of trace elements in medicine and biology. 2017;41:79-90.
12. Tinoco-Ojanguren R, Halperin DC. Poverty, production, and health: inhibition of erythrocyte cholinesterase via occupational exposure to organophosphate insecticides in Chiapas, Mexico. Archives of Environmental Health: An International Journal. 1998;53(1):29-35.
13. Ahmad KR, Tahir MZ, Khan SY, Tahir HM, Raees K, Arshad M, et al. Effects of diazinon on the ovarian micro-anatomical and micrometric parameters of pregnant mice. African Journal of Biotechnology. 2011;10(65):14656-62.
14. Sarhan O, Al-Sahhaf Z. Histological and biochemical effects of diazinon on liver and kidney of rabbits. Life Science Journal. 2011;8(4):1183-9.
15. Burgess P, Harper C, Todd GD, Wohlers D. Toxicological profile for diazinon. 2008.
16. Savy CY, Fitchett AE, McQuade R, Gartside SE, Morris CM, Blain PG, et al. Low-level repeated exposure to diazinon and chlorpyrifos decrease anxiety-like behaviour in adult male rats as assessed by marble burying behaviour. Neurotoxicology. 2015;50:149-56.
17. Cakici O, Akat E. Effects of Oral Exposure to Diazinon on Mice Liver and Kidney Tissues. Anal Quant Cytopathol Histopathol. 2013;35:7-16.
18. El-Shenawy NS, Al-Eisa RA, El-Salmy F, Salah O. Prophylactic effect of vitamin E against hepatotoxicity, nephrotoxicity, haematological indices and histopathology induced by diazinon insecticide in mice. Current Zoology. 2009;55(3):219-26.
19. Davies DB, Holub BJ. Comparative subacute toxicity of dietary diazinon in the male and female rat. Toxicology and Applied Pharmacology. 1980;54(3):359-67.
20. Ray DE. Chronic effects of low level exposure to anticholinesterases—a mechanistic review. Toxicology letters. 1998;102:527-33.
21. Akturk O, Demirin H, Sutcu R, Yilmaz N, Koylu H, Altuntas I. The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C. Cell biology and toxicology. 2006;22:455-61.
22. Giordano G, Afsharinejad Z, Guizzetti M, Vitalone A, Kavanagh TJ, Costa LG. Organophosphorus insecticides chlorpyrifos and diazinon and oxidative stress in neuronal cells in a genetic model of glutathione deficiency. Toxicology and applied pharmacology. 2007;219(2-3):181-9.
23. Li J, Bi H. Integrated strategy of network pharmacology and in vitro screening to identify mechanism of diazinon-induced hippocampal neurotoxicity. Neurotoxicology. 2022;92:122-30.
24. Li J, Sun Y, Bi H. Exploring molecular mechanisms of diazinon toxicity in HT22 hippocampal neurons through integrated miRNA and mRNA profiling. Computers in Biology and Medicine. 2024;182:109091.
Pathobiology Reserach
Volume 28, Issue 1
February 2025
Pages 57-63