Comparison of the Tumor-Suppressing Effects of Aqueous and Hydroalcoholic Extracts of Pomegranate Peel on Gastric Cancer

Document Type : Original Research

Authors
Nooshin Asadmasjedi 1
Marzieh Anam 2
Fateme Kiani 3
Zahra Behvandi 3
Haniye Shahoon 3
Marzieh Zeinvand-Lorestani 4
1 Department of Medical Laboratory Sciences, School of Paramedical Sciences, Dezful University of Medical Sciences, Dezful, Iran
2 Infectious and Tropical Disease Research Center, Dezful University of Medical Sciences, Dezful, Iran
3 Student Research Committee, Dezful University of Medical Sciences, Dezful, Iran
4 Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
10.48311/mjms.2025.109305.0
Abstract
 Background: The active compounds of pomegranate peel (PP) have different effects and their solubility varies based on their polarity. There are limited studies that thoroughly examine and compare the effects of hydroalcoholic and aqueous extracts of its components so this study will investigate which extract (aqueous or hydroalcoholic) has a greater effect on gastric cancer cells (AGS cell line).
Methods: Gastric cancer cell line (AGS) used and subjected to different concentrations of aqueous or hydroalcoholic extract of PPE, from 0.25 to 8 mg/ml then viability, and IC50 assays were performed.
Results: In our study, both aqueous and hydroalcoholic extracts of pomegranate peel exhibited anti-tumor properties against AGS cells (p ≤ 0.01). The IC50 value of the aqueous extract was determined to be 2.784 mg/mL, indicating a higher potency in inhibiting gastric cancer cell proliferation. In comparison, the hydroalcoholic extract exhibited a higher IC50 value of 3.58856 mg/mL, suggesting it is less effective at the same concentration.
Conclusion: Therefore, the aqueous extract demonstrates a stronger cytotoxic effect against gastric cancer cells compared to the hydroalcoholic extract.

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Pathobiology Reserach
Volume 28, Issue 4
Autumn 2025
Pages 31-37