Volume 21, Issue 1 (2018)                   mjms 2018, 21(1): 23-28 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sahafian M, Farzanegi P, Abbaszadeh H, Vizvari E. Effect of Caffeine and Genistein Supplement on Cardiac Levels of Nitric Oxide and Vascular Endothelial Growth Factor in Wistar Rats, Following an Acute Swimming Exercise. mjms. 2018; 21 (1) :23-28
URL: http://mjms.modares.ac.ir/article-30-22436-en.html
1- Exercise Physiology Department, Humanities Faculty, Sari Branch, Islamic Azad University, Sari, Iran
2- Exercise Physiology Department, Humanities Faculty, Sari Branch, Islamic Azad University, Sari, Iran, ساری،کیلومتر هفت جاده دریا، دانشگاه آزاد اسلامی واحد ساری، دانشکده علوم انسانی. کدپستی: 4816119318‏
Abstract:   (7379 Views)
Aims: Exercise activity and the use of herbal supplements are effective in regulating vascular contraction by influencing on angiogenesis factors. The purpose of this study was to investigate the effect of caffeine and genistein supplement on cardiac levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in wistar rats, following an acute swimming exercise.
Materials and Methods: In this experimental study, 49 male Wistar 8-10-week rats were randomly divided into 7 groups of control, saline, acute exercise, caffeine, genistein, acute swimming exercise plus caffeine, and acute swimming exercises plus genistein (7 rats in each group). Acute exercise included 90 minutes swimming. Dietary supplement groups, taked the caffeine at a rate of 5mg/kg of body weight by gavage and Genistein in the amount of 100mg/kg of body weight by intra peritoneal injection. Two hours after the end of the training session, the levels of NO and VEGF were measured by ELISA. Data were analyzed by SPSS 20 software using one-way ANOVA.
Findings: 90 minutes swimming exercise caused a significant increase in cardiac levels of NO and VEGF in acute exercise, acute exercise plus caffeine, acute exercise plus genistein in comparison to the control and saline groups (p=0.01), while supplementation of caffeine or genistein alone did not significantly increase the levels of cardiac level of these factors compared to control and saline groups.
Conclusion: The supplementation of caffeine and genistein increases the levels of NO and VEGF of the heart tissue of rats following an acute swimming session.
Full-Text [PDF 408 kb]   (968 Downloads)    
Article Type: Original Research | Subject: Sport physiology
Received: 2018/06/25 | Accepted: 2018/06/25

References
1. Momenan A, Delshad M, Mirmiran P, Ghanbarian A, Safarkhani M, Azizi F. Physical inactivity and related ‎factors in an adult tehranian population (Tehran lipid and glucose study). Iran J Endocrinol Metab. ‎‎2012;13(5):493-503. (Persian)‎ [Link]
2. Taheri Chadorneshin H, Nourshahi M, Ranjbar K. The response of vascular endothelial growth factor to ‎exhausted submaximal exercise and its relationship with VO2max. J Sport Biosci. 2010;2(7):57-75. [Persian]‎ [Link]
3. Ghardashi Afousi AR, Gaeini A, Gholami Borujeni B. The effect of aerobic interval training on endothelial ‎vasculature function in type 2 diabetes patient. Iran J Rehabil Nurs. 2016;2(3):27-39. [Persian]‎ [Link]
4. Jalali Z, Dabidi Roshan V. The Effect of regular endurance exercises and Galbanum supplement on vascular ‎Function during chronic hypertension in male wistar rats. Sport Biosci (Harakat). 2014;6(1):95-113. ‎‎[Persian]‎ [Link]
5. Mahrou‏ ‏M, Gaeini AA, Chobbineh S, Javidi M. Changes in stimulating factors of angiogenesis, induced by ‎progressive resistance training in diabetic rats. Iran J Diabet Metab. 2014;14(1):1-8. [Persian]‎ [Link]
6. Green DJ, Spence A, Halliwill JR, Cable NT, Thijssen DH. Exercise and vascular adaptation in asymptomatic ‎humans. Exp Physiol. 2011;96(2):57-70.‎ [Link]
7. Nourshahi M, Fayaz-Milani R, Gholamali M. Effects of acute eccentric exercise on serum vascular ‎endothelial growth factor and endostatin concentration in male wistar rats. J Sport Biomotor Sci. ‎‎2011;3(5):86-94. [Perseian]‎ [Link]
8. Fallah Mohammadi Z, Dabidi Roshan V, kanemati H. Effect of vitamin E supplementation and acute a ‎Resistance training on CPK, LDH, and plasma nitric oxide in inactive men. Olympic. 2011;19(3):35-46. ‎‎[Persian]‎ [Link]
9. Jensen L, Pilegaard H, Neufer PD, Hellsten Y. Effect of acute exercise and exercise training on VEGF splice ‎variants in human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2004;287(2):R397-402.‎ [Link] [DOI:10.1152/ajpregu.00071.2004]
10. Gu JW, Gadonski G, Wang J, Makey I, Adair TH. Exercise increases endostatin in circulation of healthy ‎volunteers. BMC Physiol. 2004;4:2.‎ [Link] [DOI:10.1186/1472-6793-4-2]
11. Czarkowska-Paczek B, Zendzian-Piotrowska M, Bartlomiejczyk I, Przybylski J, Gorski J. The influence of ‎physical exercise on the generation of TGF-β1, PDGF-AA, and VEGF-A in adipose tissue. Eur J Appl Physiol. ‎‎2011; 111(5):875-81.‎ [Link] [DOI:10.1007/s00421-010-1693-2]
12. Jafari A, Nik-Kherad J, Maleki-rad AA. Effect of short-term caffeine supplementation on downhill running ‎induced‏ ‏inflammatory response in non-athletes male. J Cell Tissue. 2012;2(4):377-85. [Persian]‎ [Link]
13. Yukawa GS, Mune M, Otani H, Tone Y, Liang XM, Iwahashi H, et al. Effects of coffee consumption on ‎oxidative susceptibility of low-density lipoproteins and serum lipid levels in humans. Biochemistry (Mosc). ‎‎2004;69(1):70-4.‎ [Link]
14. ‎Damirchi A, Rahmani-nia F, Mirzaei B, Hasan-Nia S, Ebrahimi M. Effect of caffeine on blood pressure ‎during exercise and at rest in overweight men. Iran J Endocr Metab. 2009;10(6):623-8. [Persian]‎ [Link]
15. McClaran SR, Wetter TJ. Low doses of caffeine reduce heart rate during submaximal cycle ergometry. J ‎Int Soc Sports Nutr. 2007;4:11.‎ [Link]
16. Liu D, Jiang H, Grange RW. Genistein activates the 3',5'-cyclic adenosine monophosphate signaling ‎pathway in vascular endothelial cells and protects endothelial barrier function. Endocrinology. ‎‎2005;146(3):1312-20.‎ [Link] [DOI:10.1210/en.2004-1221]
17. Liu D, Homan LL, Dillon JS. Genistein acutely stimulates nitric oxide synthesis in vascular endothelial cells ‎by a cyclic adenosine 5'-monophosphate-dependent mechanism. Endocrinology. 2004;145(12):5532-9.‎ [Link] [DOI:10.1210/en.2004-0102]
18. Poulaki V, Joussen AM, Mitsiades N, Mitsiades CS, Iliaki EF, Adamis AP. Insulin-like growth factor-I plays a ‎pathogenetic role in diabetic retinopathy. Am J Pathol. 2004;165(2):457-69.‎ [Link] [DOI:10.1016/S0002-9440(10)63311-1]
19. Mehri Alvar Y, Sayevand Z, Erfani Adab F, Heydari Moghadam R, Samavat Sharif MA, Karami S. The ‎effects of five weeks' resistance training on some vascular growth factors in sedentary men. Sport Physiol. ‎‎2016;8(29):15-30. [Persian]‎ [Link]
20. Graham DA, Rush JW. Exercise training improves aortic endothelium-dependent vasorelaxation and ‎determinants of nitric oxide bioavailability in spontaneously hypertensive rats. J Appl Physiol (1985). ‎‎2004;96(6):2088-96.‎ [Link]
21. Gavin TP, Drew JL, Kubik CJ, Pofahl WE, Hickner RC. Acute resistance exercise increases skeletal muscle ‎angiogenic growth factor expression. Acta Physiol (Oxf). 2007;191(2):139-46.‎ [Link] [DOI:10.1111/j.1748-1716.2007.01723.x]
22. Wang WQ, Lu CF, Jiang WK, Pan JF. Effects of acute exercise training and hypoxia on vascular endothelial ‎cell growth factor expression in rat gastrocnemius muscles. J Clin Rehabil Tissue Eng Res. 2009;13(50):9874-‎‎8.‎ [Link]
23. Ranjbar K, Nourshahi M, Gholamali M. The effect of acute sub-maximal endurance exercise on serum ‎angiogenic indices in sedentary men. Zahedan J Res Med Sci. 2014;16(6):58-63. [Persian]‎ [Link]
24. Czarkowska-Paczek B, Bartlomiejczyk I, Przybylski J. The serum levels of growth factors: PDGF, TGF-beta ‎and VEGF are increased after strenuous physical exercise. J Physiol Pharmacol. 2006;57(2):189-97.‎ [Link]
25. Kraus RM, Stallings HW 3rd, Yeager RC, Gavin TP. Circulating plasma VEGF response to exercise in ‎sedentary and endurance-trained men. J Appl Physiol (1985). 2004;96(4):1445-50.‎ [Link]
26. Li H, Jin SY, Son HJ, Seo JH, Jeong GB.‎‏ ‏Caffeine-induced endothelial cell death and the inhibition of ‎angiogenesis. Anat Cell Biol. 2013;46(1):57-67.‎ [Link] [DOI:10.5115/acb.2013.46.1.57]
27. Viboolvorakul S, Patumraj S. Exercise training could improve age-related changes in cerebral blood flow ‎and capillary vascularity through the upregulation of VEGF and eNOS. Biomed Res Int 2014;2014:230791.‎ [Link]
28. Jalili C, Ahmadi S, Roshankhah S, Salahshoor M. Effect of Genistein on reproductive parameter and serum ‎nitric oxide levels in morphine-treated mice. Int J Reprod Biomed (Yazd). 2016;14(2):95-102.‎ [Link] [DOI:10.29252/ijrm.14.2.95]

Add your comments about this article : Your username or Email:
CAPTCHA