Volume 22, Issue 3 (2019)                   mjms 2019, 22(3): 149-157 | Back to browse issues page

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Abedy Ghehi F, Fathi R, Abtahi N, Eivazkhani F, Bahrehbar K, Abed Heidari E, et al . Germ cells Markers Expression in Mouse Premature Ovarian Failure Model. mjms 2019; 22 (3) :149-157
URL: http://mjms.modares.ac.ir/article-30-29850-en.html
Germ cells Markers Expression in Mouse Premature Ovarian Failure Model
F. Abedy Ghehi1 , R. Fathi 2, N.S. Abtahi1 , F. Eivazkhani1 , Kh. Bahrehbar1 , E. Abed Heidari1 , S. Tavana1 , L. Rashki Ghaleno1 , H. Imani1 , L. Montazeri3
1- Embryology Department, Reproductive Biomedicine Research Center, Institute for Biology & Reproductive Medicine, Royan Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
2- Embryology Department, Reproductive Biomedicine Research Center, Institute for Biology & Reproductive Medicine, Royan Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran , rfathi79@royaninstitute.org
3- Stem Cells & Developmental Biology Department, Cell Science Research Center, Royan Institute for Stem Cell Biology & Technology, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Abstract:   (7872 Views)

Aims: Premature ovarian failure is a syndrome causing amenorrhea, infertility, and increases gonadotropin levels before age 40. The use of chemotherapy drugs can be one of the reasons that lead to this disorder. The purpose of this study was to evaluate the presence of germ cells markers in mice model of premature ovarian failure following chemotherapy drugs.
Materials and Methods: In this study, 24 mature female mice were used to create a premature ovarian failure model, different amount of cyclophosphamide and busulfan were applied (experimental groups 1 to 5). Bodyweight change, vaginal smear, morphological alternation of ovarian tissue in both experimental and control (without treatment) groups were evaluated and for the best model, hormonal evaluation (FSH, E2), and expression of germline markers (Oct4, Dazl) were examined.
Findings: Since, in the second group estrus cycle disorder, the significant decrease in weight and ovarian reserve (p˂0.05) were observed, compared to the control group, so this group was chosen as the best model. An increase in FSH level and reduction in estradiol level in the second group, compared with the control group (p˂0.05), confirmed creation of the POF model. Also, genes expression of Oct-4 and Dazl showed an increase (p˂0.05) in the second group compared with the control one.
Conclusion: The presence of germ cells markers in a mouse model of premature ovarian failure following the use of chemotherapy drugs can be a new hope in the treatment of infertility in cancer patients after chemotherapy.
 

Keywords: Premature Ovarian Failure, Ovarian Reserve, Chemotherapy, Germ Line
Full-Text [PDF 1060 kb]   (2634 Downloads)    
Article Type: Original Research | Subject: Embryology
Received: 2019/01/27 | Accepted: 2019/07/12
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