Volume 23, Issue 3 (2020)                   Pathobiol Res 2020, 23(3): 129-135 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahimkhani A, Haghighat S, Mahdavi M. Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses. Pathobiol Res. 2020; 23 (3) :129-135
URL: http://mjms.modares.ac.ir/article-30-32448-en.html
1- Department of Microbiology, Medical Sciences Branch, Islamic Azad University, Tehran, Iran
2- 2 Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran , mahdavivac@gmail.com
Abstract:   (308 Views)
Aims: The common hepatitis B vaccination was designed based on alum adjuvant. This adjuvant is failed to stimulate cellular immunity so that, optimization is necessary for better stimulation of cellular immune responses. α--tocopherol is a key component of vitamin E that is considered as immunomodulatory agent to regulate immunity in order to decrease inflammation immune responses. Therefore, in the present study, the effect of α-tocopherol on the improvement of immune responses in hepatitis B vaccination was evaluated.
Materials & Methods: commercial hepatitis B vaccine was formulated with α-tocopherol at the doses of 1, 5, and 10mg. The vaccine was injected into BALB/C mice three times two weeks apart. Ten days after the last injection, blood-drawing was performed from mice groups. The levels of IFN-γ, TNF-α, IL-4, and IL-2 cytokines and IgG total antibody, as well as IgG1 and IgG2a antibody isotypes, were measured by ELISA method.
Findings: Formulated vaccine at the dose of 10mg of α-tocopherol is more potent in the significant increase of IFN-γ, TNF-α, and IL-2 cytokines and low dose result in improvement of humoral immune response.
Conclusion: Immune response of vaccine formulated with α-tocopherol was dose-dependent and at the dose of 10mg demonstrated a significant increase of Th1 response and at the dose of 1mg result in increase of antibody responses.
Full-Text [PDF 415 kb]   (86 Downloads)    
Article Type: Original Research | Subject: Immunology and Allergy
Received: 2019/05/19 | Accepted: 2020/08/4 | Published: 2020/09/20
* Corresponding Author Address: Recombinant Vaccine Research Center, Tehran University of Medical Sciences, 16 Azar Street, Tehran, Iran

References
1. Bustamante J, Rimola A, Ventura PJ, Navasa M, Cirera I, Reggiardo V, et al. Prognostic significance of hepatic encephalopathy in patients with cirrhosis. J Hepatol. 1999;30(5):890-5. [Link] [DOI:10.1016/S0168-8278(99)80144-5]
2. Trépo Ch, Chan HL, Lok A. Hepatitis B virus infection. Lancet. 2014;384(9959):2053-63. [Link] [DOI:10.1016/S0140-6736(14)60220-8]
3. Bruss V, Lu XU, Thomssen R, Gerlich WH. Post‐translational alterations in transmembrane topology of the hepatitis B virus large envelope protein. EMBO J. 1994;13(10):2273-9. [Link] [DOI:10.1002/j.1460-2075.1994.tb06509.x]
4. Seeger Ch, Mason WS. Molecular biology of hepatitis B virus infection. Virology. 2015;479-480:672-86. [Link] [DOI:10.1016/j.virol.2015.02.031]
5. Wakita T, Pietschmann T, Kato T, Date T, Miyamoto M, Zhao Z, et al. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med. 2005;11(7):791-6. [Link] [DOI:10.1038/nm1268]
6. Norder H, Couroucé AM, Coursaget P, Echevarria JM, Lee SD, Mushahwar IK, et al. Genetic diversity of hepatitis B virus strains derived worldwide: Genotypes, subgenotypes, and HBsAg subtypes. Intervirology. 2004;47(6):289-309. [Link] [DOI:10.1159/000080872]
7. Cattaneo R, Will H, Schaller H. Hepatitis B virus transcription in the infected liver. EMBO J. 1984;3(9):2191-6. [Link] [DOI:10.1002/j.1460-2075.1984.tb02113.x]
8. Chen MT, Billaud JN, Sällberg M, Guidotti LG, Chisari FV, Jones J, et al. A function of the hepatitis B virus precore protein is to regulate the immune response to the core antigen. Proc Natl Acad Sci. 2004;101(41):14913-8. [Link] [DOI:10.1073/pnas.0406282101]
9. Stuyver LJ, Locarnini SA, Lok A, Richman DD, Carman WF, Dienstag JL, et al. Nomenclature for antiviral-resistant human hepatitis B virus mutations in the polymerase region. Hepatology. 2001;33(3):751-7. [Link] [DOI:10.1053/jhep.2001.22166]
10. Farrell PM, Bieri JG, Fratantoni JF, Wood RE, Di Sant'Agnese PA. The occurrence and effects of human vitamin E deficiency: A study in patients with cystic fibrosis. J Clin Investig. 1977;60(1):233-41. [Link] [DOI:10.1172/JCI108760]
11. Saberi AH, Fang Y, McClements DJ. Fabrication of vitamin E-enriched nanoemulsions: Factors affecting particle size using spontaneous emulsification. J Colloid Interface Sci. 2013;391:95-102. [Link] [DOI:10.1016/j.jcis.2012.08.069]
12. Riese P, Schulze K, Ebensen T, Prochnow B, A Guzmán C. Vaccine adjuvants: Key tools for innovative vaccine design. Curr Top Med Chem. 2013;13(20):2562-80. [Link] [DOI:10.2174/15680266113136660183]
13. Couch RB, Bayas JM, Caso C, Mbawuike IN, López CN, Claeys C, et al. Superior antigen-specific CD4+ T-cell response with AS03-adjuvantation of a trivalent influenza vaccine in a randomised trial of adults aged 65 and older. BMC Infect Dis. 2014;14(1):425. [Link] [DOI:10.1186/1471-2334-14-425]
14. Lima KM, Dos Santos SA, Rodrigues Jr JM, Silva CL. Vaccine adjuvant: It makes the difference. Vaccine. 2004;22(19):2374-9. [Link] [DOI:10.1016/j.vaccine.2003.12.030]
15. Rezaei M, Hosseini SN, Khavari-Nejad RA, Najafi F, Mahdavi M. HBs antigen and mannose loading on the surface of iron oxide nanoparticles in order to immuno-targeting: Fabrication, characterization, cellular and humoral immunoassay. Artif Cells Nanomed Biotechnol. 2019;47(1):1543-58. [Link] [DOI:10.1080/21691401.2019.1577888]
16. Radhakrishnan AK, Mahalingam D, Selvaduray KR, Nesaretnam K. Supplementation with natural forms of vitamin E augments antigen-specific TH1-type immune response to tetanus toxoid. BioMed Res Int. 2013;2013:782067. [Link] [DOI:10.1155/2013/782067]
17. Hahn T, Jagadish B, Mash EA, Garrison K, Akporiaye ET. α-Tocopheryloxyacetic acid: A novel chemotherapeutic that stimulates the antitumor immune response. Breast Cancer Res. 2011;13(1):R4. [Link] [DOI:10.1186/bcr2808]
18. Karlsson I, Borggren M, Nielsen J, Christensen D, Williams J, Fomsgaard A. Increased humoral immunity by DNA vaccination using an α-tocopherol-based adjuvant. Hum Vaccin Immunother. 2017;13(8):1823-30. [Link] [DOI:10.1080/21645515.2017.1321183]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author