Volume 13, Issue 3 (2010)                   mjms 2010, 13(3): 11-21 | Back to browse issues page

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1- Biomaterials Group, Biomedical Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
2- Department of Biotechnology, Cellular & Molecular Research Center, Iran University of Medical Science, Tehran, Iran
3- No. 424, Hafez Ave. Amirkabir university of technology, Biomedical Engineering Faculty
Abstract:   (5280 Views)
Objective: Silicon is an effective element in bone biomineralization; hence Si-substituted hydroxyapatite can be a relevant bioceramic as bone materials substitution. Materials and Methods: Stoichiometric hydroxyapatite (HA) and Si-substituted hydroxyapatite (Si-HA) with different contents of Si substitution were synthesized successfully by a hydrothermal method using Ca(NO3)2, (NH4)3PO4 or (NH4)2HPO4 and Si(OCH2CH3)4 (TEOS) as starting materials. Results: Crystalline Phases, chemical composition, microstructure and morphology of synthesized powders were investigated using X-ray diffraction (XRD), Fourier transform IR spectroscopy (FTIR), inductively coupled plasma AES (ICP-AES) and scanning electron microscopy (SEM) techniques. The results proved silicon substitution in hydroxyapatite structure and revealed that the substitution of phosphate groups by silicate groups caused some OH- loss to maintain charge balance and the lattice parameters slightly changed with respect to stoichiometric HA. Conclusion: Si-incorporation reduces the crystallites size of Si-HA and crystallinity, thus the solubility of Si-HA powders increases, and as a result Si- substitution has improved bioactivity behavior of HA. Based on in-vitro tests; soaking and incubating the specimens in simulated body fluid (SBF) and MTT assays (Dimethylthiazol assay), Si-substituted hydroxyapatite is more bioactive than pure hydroxyapatite.
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Received: 2010/05/14 | Accepted: 2010/07/24

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