Volume 23, Issue 3 (2020)                   Pathobiol Res 2020, 23(3): 149-156 | Back to browse issues page

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Hassanlou M. The Effect of Static Magnetic Field on the Rate of proIAPP Amyloid Structures Formation and the Toxicity of Amyloid Structures of Lysozyme. Pathobiol Res. 2020; 23 (3) :149-156
URL: http://mjms.modares.ac.ir/article-30-41374-en.html
Department of Cellular and Molecular Biology, Biology Faculty, Women’s University of Semnan (Farzanegan), Semnan, Iran , hassanlou@fgusem.ac.ir
Abstract:   (355 Views)
Aims: Living cells have an electrical charge created by the presence of ions and free radicals. Magnetic fields interact with ions, especially ferromagnetic materials such as iron that affect living cells. A common feature of about 20 different diseases is the aggregation of proteins in the form amyloid structure. In the current study, the effect of static magnetic field (SMF) on the formation and the toxicity of amyloid structures was investigated.
Materials & Methods: CHO cells were exposed to 6mT SMF in three consecutive days, and the effect of SMF on the formation of amyloid structures in the intrinsic proteins of these cells related to the control was investigated using thioflavin T (ThT) binding assay. The formation of amyloid structures in CHO cells expressing human ProIAPP cells was analyzed by observation of proIAPP protein aggregates linked to GFP protein. The effect of SMF on the toxicity of lysozyme oligomers on CHO and Hela cells was also compared with the controls.
Findings: Exposure of CHO cells to magnetic fields does not have a significant effect on the formation of amyloid structures in the intrinsic proteins of CHO cells and the amount of these structures in CHO cells expressing proIAPP protein but can increase the toxicity of lysozyme oligomers on CHO and Hela cells.
Conclusion: The magnetic field does not have a significant effect on the formation of amyloid structures in the 6mT SMF strength, but it adds to the toxicity of these structures.
Full-Text [PDF 911 kb]   (47 Downloads)    
Article Type: Original Research | Subject: Molecular Medicine
Received: 2020/03/18 | Accepted: 2020/08/12 | Published: 2020/09/20
* Corresponding Author Address: Biology Faculty, Women’s University of Semnan (Farzanegan), Semnan, Iran. Postal Code: 3519735117

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