Volume 23, Issue 3 (2020)                   Pathobiol Res 2020, 23(3): 137-142 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghasemi N, Falsafi S, Amini K. Evaluation the effect of sea cucumber (Holothuria leucospilota) on invA gene expression in Salmonella infantis. Pathobiol Res. 2020; 23 (3) :137-142
URL: http://mjms.modares.ac.ir/article-30-42239-en.html
1- Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran
2- Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Branch, Tehran Medical Science Islamic Azad University, Tehran, Iran , Sarvnaz_falsafi@yahoo.com
3- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract:   (369 Views)
Introduction: The invA gene plays a critical role in the pathogenicity of Salmonella infantis. Marine organisms, including sea cucumbers due to their effective secondary metabolites, have been identified and studied with compounds with antioxidant, anti-cancer, antibacterial and anti-inflammatory properties. The aim of this study is to determine effect of sea cucumber extraction (Holothuria leucospilota)  on S. infantis invA gene expression .
Material & Methods: Poultry meat was sampled. The S. infantis strains containing invA gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively.
Results: Morphological and biochemical characteristics of these bacteria were confirmed. From 450 samples, 12 S.infantis isolates were isolated. The PCR technique was used to identify the invA encoding gene. All 12 isolates have invA virulence genes. MIC was determined 256 µg/ml. The effect of sea cucumber extract on invA gene expression in S.infantis was evaluated, and the rate of change for the invA gene is estimated -1.21.
Discussion & Conclusion: According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against S. infantis
Full-Text [PDF 855 kb]   (92 Downloads)    
Article Type: Original Research | Subject: Microbiology
Received: 2020/04/20 | Accepted: 2020/08/7 | Published: 2020/09/20
* Corresponding Author Address: Faculty of Advanced Science and Technology, Tehran Medical Science Islamic Azad University, Tehran, Iran. Postal Code: 1916893813

References
1. Farouk AE, Ghouse FA, Ridzwan BH. New bacterial species isolated from Malaysian sea cucumbers with optimized secreted antibacterial activity. Am J Biochem Biotechnol. 2007;3(2):60-5. [Link] [DOI:10.3844/ajbbsp.2007.60.65]
2. Ebrahimi H, Mohebi G, Vaziri Zadeh A, Nabipour I, Nafisi Bahabadi M. Sea cucumber, ocean bioactive compounds. South Med. 2015;18(3):664-79. [Persian] [Link]
3. Jamali S, Emtiazjoo M, Timori Toolabi L, Zeinali S, Kipour S, Sardari S, et al. Antibacterial effect of natural extracts of Persian Gulf sea cucumber Holoturia. SP on three strains of Escherichia coli. Pathobiol Res. 2009;12(2):37-49. [Persian] [Link]
4. Farjami B, Nematollahi MA, Noradi Y, Irajian G, Nazemi M. Study of antibacterial effect of the extracts of the sea cucumber (Holothuria leucospilota) of Persian Gulf on the Escherichia coli. Iran J Med Microbiol. 2014;8(1):27-33. [Persian] [Link]
5. Cloeckaert A, Schwarz S. Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104. Vet Res. 2001;32(3-4):301-10. [Link] [DOI:10.1051/vetres:2001126]
6. Darwin KH, Miller VL. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clin Microbiol Rev. 1999;12(3):405-28. [Link] [DOI:10.1128/CMR.12.3.405]
7. Amini K, Zahraei Salehi T, Nikbakht G, Ranjbar R, Amini J, Ashrafganjooei SB. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. Afr J Microbiol Res. 2010;4(21):2202-10. [Link]
8. Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev. 2000;13(4):559-70. [Link] [DOI:10.1128/CMR.13.4.559]
9. Ahmadi Roudbaraki Z, Ranji N, Soltani Tehrani B. Deregulation of mexb gene in ciprofloxacin resistant isolates of pseudomonas aeruginosa treated with silibinin-encapsulated in nanoparticles. J Babol Univ Med Sci. 2017;19(11):42-9. [Persian] [Link]
10. Amavisit P, Lightfoot D, Browning GF, Markham PF. Variation between pathogenic serovars within Salmonella pathogenicity islands. J Bacteriol. 2003;185(12):3624-35. [Link] [DOI:10.1128/JB.185.12.3624-3635.2003]
11. Corrêa IM, Pereira LQ, Silva IG, Altarugio R, Smaniotto BD, Silva TM, et al. Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse. Pesquisa Veterinária Brasileira. 2018;38(7):1300-6. [Link] [DOI:10.1590/1678-5150-pvb-5211]
12. Moghadam A, Nazarian Sh, Amani J. Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province. Iran J Med Microbiol. 2017;11(2):1-8. [Persian] [Link]
13. Han SR, Schindel C, Genitsariotis R, Märker-Hermann E, Bhakdi S, Maeurer MJ. Identification of a unique Helicobacter Species by 16S rRNA gene analysis in an abdominal abscess from a patient with x-linked hypogammaglobulinemia. J Clin Microbiol. 2000;38(7):2740-2. [Link] [DOI:10.1128/JCM.38.7.2740-2742.2000]
14. Pishehvarzad F, Yousefzadi M, Kamrani E, Moini Zanjani T, Ali Ahmadi A. Antioxidant activity of extracts of two species of sea cucumber Holothuria parva and Holothuria leucospilota from the Persian Gulf, Iran. J Aquat Ecol. 2014;4(1):34-29. [Persian] [Link]
15. Esmaeili A, Shahnaghi N, Roustaeian A, Ashja Ardalan A, Bahmaei M. Investigation of antioxidant activity of nonpolar and semipolar natural compounds of the Persian Gulf Sea Cucumber (Holothuria sp.) by using Oil Stability Index (OSI) method. Iran Sci Fish J. 2009;18(1):13-20. [Persian] [Link]
16. Nazemi M, Moradi Y, Gozari M, Legzaee F, Karimpour M. Investigations of antibacterial activity of methanol and aqueous ex-tracts of the body wall of sea cucumber Holothuria leucospilota on some human pathogenic bacteria. Avicenna J Clin Med. 2016;23(1):75-82. [Persian] [Link]
17. Fey A, Eichler S, Flavier S, Christen R, Höfle MG, Guzmán CA. Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism. Appl Environ Microbiol. 2004;70(6):3618-23. [Link] [DOI:10.1128/AEM.70.6.3618-3623.2004]
18. Morshed R, Peighambari SM. Salmonella infections in poultry flocks in the vicinity of Tehran. Int J Vet Res. 2010;4(4):273-6. [Link]
19. Ranjbar R, Sarshar M, Sadeghifard N. Characterization of genetic diversity among clinical strains of Salmonella enterica serovar infantis by ribotyping method. J Adv Med Biomed Res. 2012;20(81):75-84. [Persian] [Link]
20. Rahmani M, Peighambari SM, Svendsen CA, Cavaco LM, Agersø Y, Hendriksen RS. Molecular clonality and antimicrobial resistance in Salmonella entericaserovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Vet Res. 2013;9(1):66. [Link] [DOI:10.1186/1746-6148-9-66]
21. Emadi Chashni SH, Hasanzadeh M, Bozorg Mehrifard MH, Mirzaei S. Characterization of the Salmonella isolates from backyard chickens in north of Iran, by serotyping, multiplex PCR and antibiotic resistance analysis. Arch Razi Inst. 2009;64(2):77-83. [Persian] [Link]
22. Peighambari SM, Sorahi Nobar M, Morshed R. Detection of Salmonella enterica serovar Infantis among serogroup C Salmonella isolates from poultry using PCR and determination of drug resistance patterns. Sci Res Iran Vet J. 2015;11(2):53-60. [Persian] [Link]
23. Zahraie Salehi MT, Mahzoonieh MR, Vatankhah J. Identification and diagnosis of Salmonella in bovine feces using polymerase chain reaction (PCR). J Vet Res Univ Tehran. 2006;61(3):243-7. [Persian] [Link]
24. Ali Gholi R, Ahmadi M, Alizadeh F, Sina R. Searching for Salmonella by InvA gene proliferation and bacterial culture in uromiexic fecal stools. Iran J Vet Med Shahid Chamran Univ Ahvaz. 2011;7(3):50-6. [Persian] [Link]
25. Kuznetsova TA, Anisimov MM, Popov AM, Baranova SI, Afiyatullov S, Kapustina II, et al. A comparative study in vitro of physiological activity of triterpene glycosides of marine invertebrates of echinoderm type. Comp Biochem Physiol C Comp pharmacol. 1982;73(1):41-3. [Link] [DOI:10.1016/0306-4492(82)90165-4]
26. Salari Z, Souinezad I, Nazemi M, Yousefzadi M. Antibacterial activity of Saponin extracted from the sea cucumber (Stichopus hermanni) collected from the Persian Gulf. Iran Fish Sci Res Inst. 2018;27(1):59-69. [Persian] [Link]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author