Volume 26, Issue 2 (2023)
mjms 2023, 26(2): 69-74 |
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Ethics code: IR.TMU.REC.1395.514
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Rezaei S, Talebi Bazminabadi A, Mohabbati Mobarez A. High clarithromycin resistance of Helicobacter pylori strains isolated from naive patients. mjms 2023; 26 (2) :69-74
URL: http://mjms.modares.ac.ir/article-30-76393-en.html
URL: http://mjms.modares.ac.ir/article-30-76393-en.html
1- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,amin.talebi@modares.ac.ir
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,
Abstract: (836 Views)
Introduction
This study investigates the susceptibility and resistance of H. pylori isolates recovered from gastroduodenal patients naive to clarithromycin.
Methods
To that end, H. pylori strains were isolated from antral biopsies of pretreatment patients, and antral biopsy specimens were subsequently cultured. Presumptive H. pylori colonies were also confirmed on enriched Brucella agar by biochemical tests, including catalase, oxidase, rapid urease, and the standard polymerase chain reaction (PCR) method. The antimicrobial susceptibility testing was performed by standard disk diffusion methods according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Then, the Epsilometer test (E-test) was used to determine the minimal inhibition concentration (MIC).
Results
Of 180 samples, 80 (44%) were positive for urease and were included for further analysis. 65 were also positive in culture base method. The sensitivity test indicated a 23% resistance rate to clarithromycin among the six clarithromycin-resistant strains: four have a common form of the A2143G mutation, and two have A2142G mutation.
Conclusion
The PCR indicated that the level of resistance to clarithromycin was very similar to the resistance level in Iran.
This study investigates the susceptibility and resistance of H. pylori isolates recovered from gastroduodenal patients naive to clarithromycin.
Methods
To that end, H. pylori strains were isolated from antral biopsies of pretreatment patients, and antral biopsy specimens were subsequently cultured. Presumptive H. pylori colonies were also confirmed on enriched Brucella agar by biochemical tests, including catalase, oxidase, rapid urease, and the standard polymerase chain reaction (PCR) method. The antimicrobial susceptibility testing was performed by standard disk diffusion methods according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Then, the Epsilometer test (E-test) was used to determine the minimal inhibition concentration (MIC).
Results
Of 180 samples, 80 (44%) were positive for urease and were included for further analysis. 65 were also positive in culture base method. The sensitivity test indicated a 23% resistance rate to clarithromycin among the six clarithromycin-resistant strains: four have a common form of the A2143G mutation, and two have A2142G mutation.
Conclusion
The PCR indicated that the level of resistance to clarithromycin was very similar to the resistance level in Iran.
Article Type: Original Research |
Subject:
Microbial genotyping and molecular epidemiology
Received: 2024/08/3 | Accepted: 2024/10/6
Received: 2024/08/3 | Accepted: 2024/10/6
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