Volume 26, Issue 3 (2023)
mjms 2023, 26(3): 7-14 |
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Ethics code: IR. MODARES.REC.1399.123
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Daliri L, Talebi Bazminabadi A. Molecular detection of dupA-positive Helicobacter pylori from dental plaque of periodontal patients: A prospective study. mjms 2023; 26 (3) :7-14
URL: http://mjms.modares.ac.ir/article-30-76438-en.html
URL: http://mjms.modares.ac.ir/article-30-76438-en.html
1- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,amin.talebi@modares.ac.ir
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,
Abstract: (729 Views)
Introduction:
This study aimed to detect H. pylori genome in dental plaque samples and determine the frequency of the dupA after bacterial culture and PCR assay.
Materials and Methods:
In this prospective study, the inclusion criteria were as: age greater and equal to 20; the need for dental examination performed by a clinician, and finally no record of antibiotic uptake against H. pylori 30 days before sampling. All demographic information of included subjects such as gender, age, history of smoking, and anti-H. pylori therapy was also recorded. DNA was extracted and bacterial culturing was performed within 4 hours of sample receipt. A patient was declared positive for H. pylori when the confirmatory glmM PCR result.
Results:
Of 40 symptomatic subjects, thirty-five were H. pylori positive (87.5%). Among the H. pylori-positive subjects, 28 (80%) were dupA positive. Meantime, for the subjects in the healthy group, 15 cases were H. pylori positive, and among this population 6 patients (40%) were dupA positive (P value <0.05). According to this study, it has been shown that in both groups with a periodontal pocket depth of 3-4 mm and without this depth H. pylori was detectable in the dental plaques, but this rate was significantly higher in the patient group (P=0.023).
Conclusions:
Conclusively, the study can be the initial step in the designation of a specific bacterial gene to track the H. pylori infection and chance of associated diseases in clinical samples while discarding the endoscopic examination as the invasive method is the highest priority.
This study aimed to detect H. pylori genome in dental plaque samples and determine the frequency of the dupA after bacterial culture and PCR assay.
Materials and Methods:
In this prospective study, the inclusion criteria were as: age greater and equal to 20; the need for dental examination performed by a clinician, and finally no record of antibiotic uptake against H. pylori 30 days before sampling. All demographic information of included subjects such as gender, age, history of smoking, and anti-H. pylori therapy was also recorded. DNA was extracted and bacterial culturing was performed within 4 hours of sample receipt. A patient was declared positive for H. pylori when the confirmatory glmM PCR result.
Results:
Of 40 symptomatic subjects, thirty-five were H. pylori positive (87.5%). Among the H. pylori-positive subjects, 28 (80%) were dupA positive. Meantime, for the subjects in the healthy group, 15 cases were H. pylori positive, and among this population 6 patients (40%) were dupA positive (P value <0.05). According to this study, it has been shown that in both groups with a periodontal pocket depth of 3-4 mm and without this depth H. pylori was detectable in the dental plaques, but this rate was significantly higher in the patient group (P=0.023).
Conclusions:
Conclusively, the study can be the initial step in the designation of a specific bacterial gene to track the H. pylori infection and chance of associated diseases in clinical samples while discarding the endoscopic examination as the invasive method is the highest priority.
Article Type: Original Research |
Subject:
Infection control and prevention
Received: 2024/08/6 | Accepted: 2024/10/12
Received: 2024/08/6 | Accepted: 2024/10/12
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