Volume 26, Issue 4 (2023)
mjms 2023, 26(4): 27-32 |
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Ethics code: IR.MODARES.REC.1402.042
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Ebrahimi Varkiani M, vahdat S. The effect of adherent and non-adherent culture plates on the detachment of PMA-treated THP-1 cell line. mjms 2023; 26 (4) :27-32
URL: http://mjms.modares.ac.ir/article-30-77429-en.html
URL: http://mjms.modares.ac.ir/article-30-77429-en.html
1- Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,s_vahdat@modares.ac.ir
2- Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,
Abstract: (405 Views)
Introduction:
Due to their role in regulating inflammation, monocytes and macrophages are important immune system cells incorporated and evaluated in various in vitro and in vivo disease modeling experiments. Accordingly, investigating appropriate culture conditions to maintain the viability, phenotype, and functionality of these cells is considered in different studies. In this study, we tried to evaluate whether the type of culture plate affects the adhesion, survival, and morphology of PMA-treated monocytes.
Methods:
The THP-1 cell line was cultured in adherent or non-adherent culture plates and cells were treated with PMA small molecule to be induced into macrophages. The morphology of treated cells and the viability of detached cells were assessed three days post-induction.
Results and conclusion:
Our results showed that the morphology and viability of PMA-treated THP-1 cells were the same in both types of plates. In summary, we showed that the type of cell culture plate did not significantly affect PMA-treated THP-1 cells·
Due to their role in regulating inflammation, monocytes and macrophages are important immune system cells incorporated and evaluated in various in vitro and in vivo disease modeling experiments. Accordingly, investigating appropriate culture conditions to maintain the viability, phenotype, and functionality of these cells is considered in different studies. In this study, we tried to evaluate whether the type of culture plate affects the adhesion, survival, and morphology of PMA-treated monocytes.
Methods:
The THP-1 cell line was cultured in adherent or non-adherent culture plates and cells were treated with PMA small molecule to be induced into macrophages. The morphology of treated cells and the viability of detached cells were assessed three days post-induction.
Results and conclusion:
Our results showed that the morphology and viability of PMA-treated THP-1 cells were the same in both types of plates. In summary, we showed that the type of cell culture plate did not significantly affect PMA-treated THP-1 cells·
Article Type: Original Research |
Subject:
Immunology, Allergology and Rheumatology
Received: 2024/10/10 | Accepted: 2024/10/21
Received: 2024/10/10 | Accepted: 2024/10/21
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