Volume 27, Issue 2 (2024)                   mjms 2024, 27(2): 0-0 | Back to browse issues page

Ethics code: APRC- code IR.AJUMS.MEDICINE.REC.1401.008

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Hoseinynejad K, gholipour M, Nejad dehbashi F, radan M. Harmaline Improves Oxidative Stress and Inflammatory Markers in Human Lung Epithelial Cells Exposed to Elastase. mjms 2024; 27 (2)
URL: http://mjms.modares.ac.ir/article-30-77815-en.html
Harmaline Improves Oxidative Stress and Inflammatory Markers in Human Lung Epithelial Cells Exposed to Elastase
Khojasteh Hoseinynejad1 , Maram Gholipour2 , Freshteh Nejad dehbashi2 , Maryam Radan 3
1- Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , radan.maryam@yahoo.com
Abstract:   (862 Views)
Background: Harmaline exhibits a diverse array of pharmacological properties, including antimicrobial, antidiabetic, osteogenic, immunomodulatory, emmenagogue, and antitumor activities. The current study aimed to investigating the effect of harmaline on oxidative stress factors in lung epithelial cells exposed to elastase. Material and method: oxidative stress markers of lung epithelial cells were investigated in all cell groups including, control, H2O2, elastase and elastase plus harmaline (50, 100, 200 μm). lung epithelial cells (A549) were exposed to elastase with concentrations of 60 U/ml for 24 hours. In other groups, cells exposed to elastase were co-treated with three different doses of harmaline (50, 100 and 200 µm) for 24 hours at 37°C. Results: the results show a significant effect of harmaline's protective effect on cell viability, free radical production (ROS), malondialdehyde (MDA) and total antioxidant capacity (TAC). harmaline significantly increased the viability and TAC level in the cells exposed to elastase. Also, harmaline significantly decreased the percentage of free radicals and the MDA level in the cells exposed to elastase. Conclusion: The results obtained from this study showed a significant protective effect of harmaline on cell viability through increases in antioxidant defense system. Therefore, harmaline, can probably considered as a therapeutic strategy to prevent or treatment of lung diseases.
 
Keywords: Lung Epithelial Cells, Respiratory System, Oxidative Stress, Harmaline
     
Article Type: Original Research | Subject: Pharmaceutical Science
Received: 2024/11/3 | Accepted: 2024/11/12
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