Volume 13, Issue 3 (2010)                   mjms 2010, 13(3): 63-81 | Back to browse issues page

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Vazifeh Shiran N, Soleimani M, Mortazavi Y, Kaviani S, Aboulghasemi H, Nikougoftar M. 3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC). mjms. 2010; 13 (3) :63-81
URL: http://mjms.modares.ac.ir/article-30-8162-en.html
1- Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Associated Professor, Department of Pathology, Faculty of Medicine, Zanjan University of Medical Sciences
3- Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
Abstract:   (4657 Views)
Objective: Allogeneic transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (MPC)- unrestricted somatic stem cells (USSC)- was incorporated in an attempt to expand CD34+ cells from UCB. To provide a similar environment in vitro, we coated DBM scaffold with USSC cells as the matrix for support UCB-CD34+ cells growth. Materials and Methods: Human placenta USSC was isolated and characterized by morphologic and immunophenotypical analysis. UCB CD34+ cells were expanded by coculture with placental USSC in 2D and 3D environment. Suitable aliquots of cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture (LTC-IC) output. Results: Ex vivo expansion of UCB hematopoietic cells, when cultured in different 2D conditions and 3D condition for 3 weeks, was significantly enhanced, the total cell count increased within the 28-day period. For total CFC, the highest CFC expansion was observed at day 14. Flow cytometry analysis of the percentage of CD34+ cells showed a decline in USSC cocultures in 2D and 3D condition at 3 weeks. Conclusion: These results strongly suggest that human USSC may be a suitable feeder layer for expansion of hematopoietic progenitors from UCB in vitro and USSC- coated DBM can therefore provide an ex vivo mimicry of bone marrow by enhancing of surface/ volume ratio and feeder layers,recapitulate the desired niche, and provide a suitable environment for stem cell expansion and differentiation.
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Received: 2010/03/3 | Accepted: 2010/09/21

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