Volume 12, Issue 3 (2009)                   mjms 2009, 12(3): 1-8 | Back to browse issues page

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Hadjati J, Razavi A, Jamali A, Amini Sardrod A, Motamedi M, Shojaee S et al . Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation. mjms. 2009; 12 (3) :1-8
URL: http://mjms.modares.ac.ir/article-30-8184-en.html
1- Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Immunology, Faculty of Paramedicene , Tehran University of Medical Sciences, Tehran, Iran
4- Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5- Department of Parasithology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (4456 Views)
Objective: The aim of the present study was to investigate the effect of protein and DNA components of Toxoplasma gondii on maturation of dendritic cells and their efficiency in IL-12 production and proliferation of T cells. Materials and Methods: for DC generation, Bone marrow cells were cultured in the presence of GM-CSF and IL-4 for 5 days. Tumor lysate and protein or DNA components of Toxoplasma gondii were added to the culture media and incubated for another 2 days. LPS was added as control for DC maturation. Proliferation of T cells were determined by MLR and IL-12 production was measured by ELISA kit. Maturation of dendritic cell were determined by flowcytometry. Results: DCs treatment with protein components of Toxoplasma gondii caused a significant increase in IL-12 production and proliferation of T cells (P<0.001). Conclusions: Different compositions of microbial body like protein and DNA components of Toxoplasma gondii can cause augmentation of antigen presentation capacity of DC and their IL-12 production capability. Among these components the protein was more effective as compared to DNA.
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Received: 1901/12/14 | Accepted: 1901/12/14

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