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Showing 2 results for Moazeni

Mohammad Hossein Yadegari, Zahra Nasrollahi, Seyed Mohammad Moazeni,
Volume 12, Issue 3 (9-2009)
Abstract

Objective: In this study the susceptibility of Candida albicans to inhibitory effect of polyphenols under varying time (24 and 48 hours) conditions were evaluated. Materials and Methods: Green tea leaf polyphenols were extracted and analyzed by chromatography. Among polyphenols, Catechin showed stronger antifungal activity against C. albicans PTCC-5027. Catechin's MIC90 (The concentration of Catechin causing 90% growth inhibition of tested strain of C. albicans) and MFC (The minimum antifungal susceptibility of Catechin) were determined by Macro dilution test and calculation after 24 and 48 hours. Results: The antifungal activity of Catechin was time dependent. Catechin's MIC for 0.5×10³, 1×10³ and 2×10³ cells/ml was 12.5, 25 and 100 mg/ml after 24h respectively. The results after 48h for 0.5×10³, 1×10³ and 2×10³ cells/ml were 6.25, 12.5 and 25 mg/ml respectively. Fluconazol was tested on C. albicans PTCC-5027 and the results indicated that this strain of Candida is fluconazol resistant. Data shown are from three separate experiments and were analysed statistically. Conclusion: C. albicans PTCC-5027 is fluconazol resistant, however green tea leaf polyphenols especially Catechin could inhibit the growth of this yeast at MIC and MFC concentrations. On the basis of the obtained results the green tea leaf contains effective antifungal components. Since the common and generic antifungal drugs possess some side effects and also there is an increasing drug resistance, it is hoped that consumption of herbal drugs may help to cure fungal diseases and to avoid the side effects of antimycotics as a good replacement.

Volume 13, Issue 1 (3-2022)
Abstract

Investigation of factors affecting endothelial cell proliferation is an essential part of angiogenesis studies. Given the importance of inhibiting angiogenesis in the treatment of cancers, and due to the side effects and high cost of anti-angiogenic drugs such as Avastin, the use of physical agents to aid in treatment and reduce the need for high doses of the drug is noteworthy. Magnetic fields are of interest due to their long-distance and non-invasive effects, and many studies have been conducted on their effects on biological phenomena, including angiogenesis, with inconsistent results. In the present study, the effect of a 2 mT alternating magnetic field with a frequency of 200 Hz and Austin on the proliferation of human umbilical vein endothelial cells (HUVEC) was investigated. Cells were treated for 48 hours under a mixture of 50 μg/ml solution of vascular endothelial growth factor (VEGEF) and Avastin at concentrations (zero (drug control), 50, 100, 200 and 400 μg/ml) as well as field treatment groups for They were exposed to magnetic fields for 3, 6, 12, 24 and 48 hours. Then, cell proliferation was assessed using Alamar Blue colorimetric test. Data were analyzed by three-way analysis of variance. According to the findings, the exposure times of 12, 24 and 48 hours showed a significant reduction in cell proliferation compared to the control group, but this difference was not significant in the 3 and 6 hour treatments. Also, the degree of interaction of these factors with each other on HUVEC proliferation was investigated.

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